Hetero/Homo-Complexes of Sucrose Transporters May Be a Subtle Mode to Regulate Sucrose Transportation in Grape Berries
| Autor: | Jing Yan, Yumeng Cai, Han Gao, Qingyong Meng, Wenrui Tu, Ling Yin, Wenjie Dong, Yali Zhang, Zhefang Deng, Jie Wang, Jinxu Xu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Sucrose
QH301-705.5 functional variation different varieties functional regulation Article Catalysis oligomerization Inorganic Chemistry Structure-Activity Relationship chemistry.chemical_compound sucrose transporter Vitis Biology (General) Physical and Theoretical Chemistry Sugar QD1-999 Molecular Biology Spectroscopy Plant Proteins Chemistry Organic Chemistry Membrane Transport Proteins food and beverages Biological Transport Transporter General Medicine Sucrose transport Yeast Computer Science Applications Membrane protein Biochemistry Fruit Phloem Protein Multimerization Function (biology) |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 21 International Journal of Molecular Sciences, Vol 22, Iss 12062, p 12062 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms222112062 |
| Popis: | The sugar distribution mechanism in fruits has been the focus of research worldwide however, it remains unclear. In order to elucidate the relevant mechanisms in grape berries, the expression, localization, function, and regulation of three sucrose transporters were studied in three representative Vitis varieties. Both SUC11 and SUC12 expression levels were positively correlated with sugar accumulation in grape berries, whereas SUC27 showed a negative relationship. The alignment analysis and sucrose transport ability of isolated SUCs were determined to reflect coding region variations among V. vinifera, V. amurensis Ruper, and V. riparia, indicating that functional variation existed in one SUT from different varieties. Furthermore, potentially oligomerized abilities of VvSUCs colocalized in the sieve elements of the phloem as plasma membrane proteins were verified. The effects of oligomerization on transport properties were characterized in yeast. VvSUC11 and VvSUC12 are high-affinity/low-capacity types of SUTs that stimulate each other by upregulating Vmax and Km, inhibiting sucrose transport, and downregulating the Km of VvSUC27. Thus, changes in the distribution of different SUTs in the same cell govern functional regulation. The activation and inhibition of sucrose transport could be achieved in different stages and tissues of grape development to achieve an effective distribution of sugar. |
| Databáze: | OpenAIRE |
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