Glucose-6-phosphate dehydrogenase and NADPH-consuming enzymes in the rat olfactory bulb
| Autor: | Paola Ferri, Paolo Del Grande, Paolino Ninfali, Ralf Dringen, Enrica Biagiotti |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Glutathione reductase Dehydrogenase Biology Glucosephosphate Dehydrogenase medicine.disease_cause Rats Sprague-Dawley Cellular and Molecular Neuroscience chemistry.chemical_compound Olfactory nerve medicine Glucose-6-phosphate dehydrogenase Animals NADPH-Ferrihemoprotein Reductase chemistry.chemical_classification Neurons NADPH Dehydrogenase Immunohistochemistry Olfactory Bulb Olfactory bulb Bulb Rats Oxidative Stress Enzyme Glutathione Reductase chemistry Biochemistry Energy Metabolism Oxidative stress NADP |
| Zdroj: | Journal of neuroscience research. 80(3) |
| ISSN: | 0360-4012 |
| Popis: | The resistance to oxidative stress is a multifactorial reaction involving the clustering of transcriptionally regulated genes. Because glucose-6-phosphate dehydrogenase (G6PD), the principal enzyme responsible for reducing power, is highly expressed in the olfactory bulb (OB), it is of interest to verify whether other enzymes utilizing NADPH are also highly expressed. The level and localization of G6PD- and NADPH-consuming enzymes, such as NADPH-cytochrome P450 oxidoreductase (P450R), glutathione reductase (GR), and NADPH-diaphorase (NADPH-d), were analyzed in the rat olfactory bulb (OB) by quantitative histochemistry and immunohistochemistry. The highest concentration of G6PD, P450R, and GR was observed in the olfactory nerve layer (ONL), suggesting a correlation in the expression of these enzymes at the gene level. Correlation in staining intensity between G6PD and NADPH-d activities occurred only in part of the ONL, some glomeruli, and scattered periglomerular cells. This peculiar distribution of NADPH-d could reflect a spatial patterning of the nose to bulb projections. Taken together, these results indicate that G6PD expression in the ONL could be related to the importance of generating a substantial supply of NADPH to sustain the detoxifying systems represented by GR and P450R reactions and, only in discrete zones, by NADPH-d activity. |
| Databáze: | OpenAIRE |
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