LC-MS/MS Validation of a Residue Analysis Method for Penicillin G and Its Metabolites in Commercial Orange Juice
| Autor: | Jo-Marie Cook, Fadi Aldeek, Matthew Standland, Walter Hammack, Ghislain Gerard, Daniele Canzani, Mark R. Crosswhite |
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| Rok vydání: | 2017 |
| Předmět: |
Analyte
medicine.drug_class Antibiotics 02 engineering and technology Orange (colour) Tandem mass spectrometry 01 natural sciences Analytical Chemistry Tandem Mass Spectrometry medicine Environmental Chemistry Food science Chromatography High Pressure Liquid Pharmacology Orange juice Chromatography Chemistry 010401 analytical chemistry Penicillin G 021001 nanoscience & nanotechnology 0104 chemical sciences Fruit and Vegetable Juices Penicillin Citrus greening disease 0210 nano-technology Agronomy and Crop Science Citrus × sinensis Citrus sinensis Food Science medicine.drug |
| Zdroj: | Journal of AOAC INTERNATIONAL. 100:189-197 |
| ISSN: | 1944-7922 1060-3271 |
| Popis: | Florida citrus depends on a breakthrough in the fight against citrus greening disease. Of the antibiotics used to treatthis disease, penicillin G has been one of the most effective. Because orange fruit grown in the state of Florida are mainly used to produce orange juice, we have validated an ultra-HPLC tandem MS method to screen for penicillin G and its metabolites (penillic and penilloic acids) at the chemical residue level after treatment. In this method, three spike levels (0.25, 1, and 20 ng/g) were tested in triplicate. Absolute recoveries for penillic and penilloic acids were 60–75% depending on the matrix used, whereas corrected recoveries of penicillin G using an isotopically labeled internal standard were ~100%. Two product ion transitions per analyte were required for identification, which contributes to a high degree of selectivity. |
| Databáze: | OpenAIRE |
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