Comparison of Four SARS-CoV-2 Neutralization Assays
| Autor: | Annika Rössler, André Volland, Dorothee von Laer, Albert Falch, Janine Kimpel, Lydia Riepler, Wegene Borena |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viruses 030106 microbiology Immunology lcsh:Medicine medicine.disease_cause Article Neutralization 03 medical and health sciences neutralization assay Drug Discovery medicine Pharmacology (medical) neutralizing antibodies skin and connective tissue diseases Coronavirus Pharmacology biology Chemistry SARS-CoV-2 lcsh:R fungi In vitro toxicology virus diseases Antigen binding biology.organism_classification Virology body regions Titer 030104 developmental biology Infectious Diseases pseudotype virus Vesicular stomatitis virus biology.protein Antibody |
| Zdroj: | Vaccines Volume 9 Issue 1 Vaccines, Vol 9, Iss 13, p 13 (2021) |
| ISSN: | 2076-393X |
| DOI: | 10.3390/vaccines9010013 |
| Popis: | Neutralizing antibodies are a major correlate of protection for many viruses including the novel coronavirus SARS-CoV-2. Thus, vaccine candidates should potently induce neutralizing antibodies to render effective protection from infection. A variety of in vitro assays for the detection of SARS-CoV-2 neutralizing antibodies has been described. However, validation of the different assays against each other is important to allow comparison of different studies. Here, we compared four different SARS-CoV-2 neutralization assays using the same set of patient samples. Two assays used replication competent SARS-CoV-2, a focus forming assay and a TCID50-based assay, while the other two assays used replication defective lentiviral or vesicular stomatitis virus (VSV)-based particles pseudotyped with SARS-CoV-2 spike. All assays were robust and produced highly reproducible neutralization titers. Titers of neutralizing antibodies correlated well between the different assays and with the titers of SARS-CoV-2 S-protein binding antibodies detected in an ELISA. Our study showed that commonly used SARS-CoV-2 neutralization assays are robust and that results obtained with different assays are comparable. |
| Databáze: | OpenAIRE |
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