Expression profiles of types 2 and 3 iodothyronine deiodinase genes in relation to vitellogenesis in a tropical damselfish, Chrysiptera cyanea
| Autor: | Yuki Takeuchi, Se-Jae Kim, Satoshi Imamura, Sung-Pyo Hur, Nina Wambiji, Shingo Udagawa, Akihiro Takemura, Angka Mahardini, Dinda Rizky |
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| Rok vydání: | 2019 |
| Předmět: |
Thyroid Hormones
food.ingredient Deiodinase Hypothalamus 030209 endocrinology & metabolism Ovary Iodide Peroxidase 03 medical and health sciences Vitellogenin 0302 clinical medicine Endocrinology food Yolk medicine Animals Tissue Distribution RNA Messenger Phylogeny 030304 developmental biology 0303 health sciences Tropical Climate Triiodothyronine biology Chrysiptera cyanea Gene Expression Profiling Vitellogenesis biology.organism_classification Cell biology Perciformes medicine.anatomical_structure Iodothyronine deiodinase biology.protein Animal Science and Zoology Female |
| Zdroj: | General and comparative endocrinology. 285 |
| ISSN: | 1095-6840 |
| Popis: | Thyroid hormone (TH) is involved in regulating the reproduction of vertebrates. Its physiological action in the target tissues is due to the conversion of TH by iodothyronine deiodinases. In this study, we aimed to clone and characterize type 2 (sdDio2) and type 3 (sdDio3) of the sapphire devil Chrysiptera cyanea, a tropical damselfish that undergoes active reproduction under long-day conditions, and to study the involvement of THs in the ovarian development of this species. When the cDNAs of sdDio2 and sdDio3 were partially cloned, they had deduced amino acid sequences of lengths 271 and 267, respectively, both of which were characterized by one selenocysteine residue. Real-time quantitative PCR (qPCR) revealed that both genes are highly expressed in the whole brain, and sdDio2 and sdDio3 are highly transcribed in the liver and ovary, respectively. In situ hybridization analyses showed positive signals of sdDio2 and sdDio3 transcripts in the hypothalamic area of the brain. Little change in mRNA abundance of sdDio2 and sdDio3 in the brain was observed during the vitellogenic phases. It is assumed that simultaneous activation and inactivation of THs occur in this area because oral administration of triiodothyronine (T3), but not of thyroxine (T4), upregulated mRNA abundance of both genes in the brain. The transcript levels of sdDio2 in the liver and sdDio3 in the ovary increased as vitellogenesis progressed, suggesting that, through the metabolism of THs, sdDio2 and sdDio3 play a role in vitellogenin synthesis in the liver and yolk accumulation/E2 synthesis in the ovary. Taken together, these results suggest that iodothyronine deiodinases act as a driver for vitellogenesis in tropical damselfish by conversion of THs in certain peripheral tissues. |
| Databáze: | OpenAIRE |
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