Identification of Protease-Producing Halophilic Bacteria Isolated from Salt-Pond Soil
| Autor: | Hidayatun Muyasharoh, Jauharotul Afifah, Rahmi Nurdiani, Asep Awaludin Prihanto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
food.ingredient
Bacillus amyloliquefaciens medicine.medical_treatment proteolytic molecular salt bacillus amyloliquefaciens strain ub_c tuban Aquatic Science Biology lcsh:Aquaculture. Fisheries. Angling law.invention proteolytic Bacillus amyloliquefaciens strain UB_C lcsh:Oceanography food law medicine Agar salt lcsh:GC1-1581 Food science molecular Ecology Evolution Behavior and Systematics lcsh:SH1-691 chemistry.chemical_classification Protease Strain (chemistry) Tuban fungi biology.organism_classification 16S ribosomal RNA Gram staining Enzyme chemistry Animal Science and Zoology Bacteria Food Science |
| Zdroj: | Jurnal Ilmiah Perikanan dan Kelautan; Vol. 12 No. 1 (2020): JURNAL ILMIAH PERIKANAN DAN KELAUTAN; 181-187 Jurnal Ilmiah Perikanan dan Kelautan, Vol 12, Iss 1, Pp 181-187 (2020) |
| ISSN: | 2085-5842 2528-0759 |
| Popis: | Highlights The halophilic bacteria were isolated from salt-pond soil. Isolate C2 was identified as the best protease producer. Isolate C2 was identified as B. amyloliquefaciens strain UBC Abstract Protease is an important enzyme for various industries, such as pharmaceuticals, leather production, meat processing, protein hydrolyzate, food products, and waste processing industries. This study aimed to isolate and identify protease-producing bacteria isolated from salt-pond soil at the Tuban Regency, East Java, Indonesia. Bacterial communities in the soil samples were firstly isolated from the soil samples by culture-dependent technique on Luria Bertani agar enriched with 5% NaCl. Thereafter, bacterial colonies that grew on the media were purified and screened for their protease production using a skim-milk agar. The bacterial colony which produced protease was further identified using phenotypic (gram staining) and genotypic assays (the 16S rDNA sequence). The result showed that one isolate out of six (isolate C2) obtained from the soil sample was observed to produce a protease enzyme. Based on its 16S rDNA sequence, the isolate was identified as Bacillus amyloliquefaciens strain UBC. These results suggest that B. Amyloliquefaciens strain UBC is a salt-tolerant bacterium (halophilic bacteria) which has the potential to be further developed for protease-producing biological agents. |
| Databáze: | OpenAIRE |
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