Increased primary amine oxidase expression and activity in white adipose tissue of obese and diabetic db−/− mice
| Autor: | Jeremie Boucher, Christian Carpéné, Josep Mercader, Zsuzsa Iffiú-Soltész, Danielle Daviaud |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
Amine oxidase medicine.medical_specialty AOC3 Adipose Tissue White Mice Obese Adipose tissue White adipose tissue Diabetes Complications Mice Internal medicine Diabetes mellitus medicine Animals Obesity Biological Psychiatry Mice Knockout Messenger RNA Leptin receptor Chemistry Glucose transporter medicine.disease Mice Mutant Strains Up-Regulation Enzyme Activation Psychiatry and Mental health Endocrinology Neurology Female Amine Oxidase (Copper-Containing) Neurology (clinical) Cell Adhesion Molecules |
| Zdroj: | Journal of Neural Transmission. 118:1071-1077 |
| ISSN: | 1435-1463 0300-9564 |
| DOI: | 10.1007/s00702-011-0586-9 |
| Popis: | The major form of primary amine oxidase expressed in adipose tissue (AT) is encoded by AOC3 gene and is known as semicarbazide-sensitive amine oxidase, identical to vascular adhesion protein-1 (SSAO/VAP-1). Exogenous substrates of SSAO/VAP-1 (e.g. benzylamine) stimulate glucose transport in adipocytes and improve glucose tolerance when injected in diabetic rodents. Numerous reports on the circulating, soluble SSAO/VAP-1 have univocally evidenced an increase in diabetic conditions. However, only scarce studies have investigated whether obesity and/or diabetes is accompanied with variations of AOC3 expression in AT. Therefore, we compared the SSAO/VAP-1 content in different fat depots of db-/- mice (lacking leptin receptor and being hyperphagic, diabetic and obese) and db+/- littermates (normoglycemic and lean). AOC3 expression was increased in perigonadal and subcutaneous AT of db-/- mice, while the maximal velocity of benzylamine oxidation (V (max), expressed as pmoles of hydrogen peroxide produced/min/mg protein) increased only in the latter. Indeed, the relative abundance of primary amine oxidase was increased in subcutaneous AT of db-/- mice at all the levels: mRNA, protein and activity. While considering the overall capacity to oxidise amines contained in each depot, there was an increase in the hypertrophic fat pads of the obese db-/- mice, irrespective of their anatomical location, as a result of their dramatically larger mass than in lean db+/- control. Such higher amount of AT-bound primary amine oxidase warrants further studies to determine whether SSAO/VAP-1 inhibition or activation may be useful in treating metabolic diseases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink