Contrasting effects of fluoroquinolone antibiotics on the expression of the collagenases, matrix metalloproteinases (MMP)-1 and -13, in human tendon-derived cells
| Autor: | R L Harrall, Brian L. Hazleman, V. A. Curry, Graham P. Riley, Anthony N. Corps |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Nalidixic acid
Biology Achilles Tendon Gene Expression Regulation Enzymologic Microbiology Anti-Infective Agents Rheumatology Matrix Metalloproteinase 13 medicine Humans Pharmacology (medical) Collagenases RNA Messenger Cells Cultured Norfloxacin Regulation of gene expression Messenger RNA Reverse Transcriptase Polymerase Chain Reaction Interleukin Molecular biology Matrix Metalloproteinases Ciprofloxacin Collagenase Ofloxacin Matrix Metalloproteinase 1 Fluoroquinolones medicine.drug |
| Zdroj: | Rheumatology. 44:1514-1517 |
| ISSN: | 1462-0332 1462-0324 |
| DOI: | 10.1093/rheumatology/kei087 |
| Popis: | Objectives. Fluoroquinolone antibiotics may cause tendon pain and rupture. We reported previously that the fluoroquinolone ciprofloxacin potentiated interleukin (IL)-1b-stimulated expression of matrix metalloproteinases (MMP)-3 and MMP-1 in human tendon-derived cells. We have now tested additional fluoroquinolones and investigated whether they have a similar effect on expression of MMP-13. Methods. Tendon cells were incubated for two periods of 48 h with or without fluoroquinolones and IL-1b. Total ribonucleic acid (RNA) was assayed for MMP messenger RNA by relative quantitative reverse transcriptase polymerase chain reaction, with normalization for glyceraldehyde-3-phosphate dehydrogenase mRNA. Samples of supernatant medium were assayed for MMP output by activity assays. Results. MMP-13 was expressed by tendon cells at lower levels than MMP-1, and was stimulated typically 10- to 100-fold by IL-1b. Ciprofloxacin, norfloxacin and ofloxacin each reduced both basal and stimulated expression of MMP-13 mRNA. In contrast, ciprofloxacin and norfloxacin increased basal and IL-1b-stimulated MMP-1 mRNA expression. Both the inhibition of MMP-13 and the potentiation of MMP-1 expression by fluoroquinolones were accompanied by corresponding changes in IL-1b-stimulated MMP output. The non-fluorinated quinolone nalidixic acid had lesser or no effects. Conclusions. Fluoroquinolones show contrasting effects on the expression of the two collagenases MMP-1 and MMP-13, indicating specific effects on MMP gene regulation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|