Combined maceration procedure permits advanced microsurgical dissection of Thiel-embalmed specimens
| Autor: | Hannes Bangerter, Valerie Schwartz, Raphael Röthlisberger, Susanne Boemke, Michael D. Müller, Mathias Bergmann, Valentin Djonov |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microsurgery Lumbosacral Plexus Polyurethanes Autonomic Nervous System Nitric Acid 03 medical and health sciences 0302 clinical medicine Venules Organ specific Cadaver Humans Medicine Nerve Tissue 610 Medicine & health Microdissection Surgical microscope Embalming Hypogastric Plexus business.industry Dissection Water Vascular casting General Medicine Combined procedure Anatomy Sacral plexus Arterioles Postmortem Changes Blood Vessels 570 Life sciences biology 030101 anatomy & morphology business Relevant information 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft |
| DOI: | 10.1016/j.aanat.2016.10.008 |
| Popis: | Introduction Due to the realistic colour, texture conservation and preservation of biomechanical properties, Thiel-embalming is becoming the main embalming procedure for clinical courses and research based on human cadaver material. The aim of this study is to establish a new procedure that allows advanced microdissection of small vessels and intraorganic nerves in Thiel-embalmed material. Material and methods/results After a classical gross anatomical dissection, human hemipelves underwent repetitive application of 3 consecutive steps: (i) maceration with alloy of nitric acid and MiliQ water 1:10 for 24–48 h. (ii) Immersion: the hemipelves were rinsed under tap water for 20–30 min. and placed in a water bath for 1 h. The nerves become more prominent due to the swelling and increased water content. (iii) microdissection under surgical microscope. To facilitate the organ visualization perfusion with polyurethane (Pu4ii, VasQtec ® , Switzerland) in red/blue for arteries/veins respectively has been performed. Conclusion By using the proposed procedure, we performed satisfactory microdissection on Thiel-embalmed samples. The combination with polyurethane vascular casting permits visualization of small arterioles and venules in a range of 20–25 μm. The method is very suitable for demonstration of somatic and vegetative nerves. Branches of the sacral plexuses and autonomic nerves from the superior and inferior hypogastric plexus have been tracked up to the smallest intraorganic branches in a range of 12.5–15 μm. In conclusion, the established combined procedure offers a new possibility for advanced microdissection, which will allow acquisition of clinically relevant information about organ specific micro- vascularization and innervation. |
| Databáze: | OpenAIRE |
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