Assessment of diagnostic accuracy of Gene Xpert MTB/RIF in diagnosis of suspected retreatment pulmonary tuberculosis patients
| Autor: | Amany Shaker, Takwa E. Meawed |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Tuberculosis 030106 microbiology Diagnostic accuracy Mycobacterium tuberculosis 03 medical and health sciences Pulmonary tuberculosis Internal medicine medicine polycyclic compounds lcsh:RC705-779 GeneXpert MTB/RIF Ziehl–Neelsen smear biology business.industry MGIT 960 lcsh:Diseases of the respiratory system biology.organism_classification medicine.disease bacterial infections and mycoses Virology Multiple drug resistance Sputum medicine.symptom business Gene Xpert MTB/RIF Rifampicin medicine.drug |
| Zdroj: | Egyptian Journal of Chest Disease and Tuberculosis, Vol 65, Iss 3, Pp 637-641 (2016) |
| ISSN: | 0422-7638 |
| Popis: | Background Retreatment cases of tuberculosis are reemerging as public and global health concerns, not only for dissemination of infection but for their multidrug resistant behavior as well, comprising an important challenge for National Tuberculosis Control Programs. Gene Xpert MTB/RIF is an automated molecular test for synchronized detection of tuberculosis and rifampicin resistance, recommended by the World Health Organization. This study was designed to assess the performance of single-sputum Gene Xpert MTB/RIF assay against the Ziehl–Neelsen smear, traditional culture and MGIT 960 in diagnosing suspected retreatment pulmonary tuberculosis cases and rifampicin resistance. Subjects and methods Fifty-eight patients were enrolled in this cross-sectional study from two Egyptian tertiary care hospitals. The patients were selected fulfilling clinical criteria of suspected retreatment TB. Early-morning sputum specimens were collected, decontamination liquefaction technique was done, the specimen was divided into four portions, the first and second portions were for the Ziehl–Neelsen smear and cultivation on the Lowenstein Jensen media. The third portion was processed in Gene Xpert MTB/RIF. The fourth part was used for confirmation of rifampicin susceptibility testing results by MGIT 960. Results Gene Xpert MTB/RIF showed sensitivity, specificity and accuracy of 98.15%, 75% and 96.55% respectively in detection of mycobacterium tuberculosis. Regarding rifampicin mono-resistance, it carried the best sensitivity and specificity of 100% equal to that of reference test MGIT 960. Conclusion Gene Xpert MTB/RIF is a sensitive, specific and accurate test for both diagnosis of retreatment MTB and rifampicin resistance. Finally the authors recommend restandardization of reference test with Gene Xpert MTB/RIF to obtain more valid results. |
| Databáze: | OpenAIRE |
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