Further evidence that genotypically closely related strains of Legionella pneumophila can express different serogroup specific antigens
| Autor: | Nicholas A. Saunders, Nivedita Doshi, A. G. Taylor, A. Haththotuwa, T. G. Harrison |
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| Rok vydání: | 1992 |
| Předmět: |
DNA
Bacterial Microbiology (medical) clone (Java method) Genotype Restriction Mapping Microbiology Legionella pneumophila Restriction fragment Polymorphism (computer science) Humans Typing Serotyping Genetics Antigens Bacterial biology Reproducibility of Results General Medicine biology.organism_classification Bacterial Typing Techniques Restriction enzyme biology.protein Restriction fragment length polymorphism DNA Probes Polymorphism Restriction Fragment Length |
| Zdroj: | Journal of Medical Microbiology. 37:155-161 |
| ISSN: | 1473-5644 0022-2615 |
| Popis: | Summary The relationship between serogroup and genotype of Legionella pneumophila strains was investigated by restriction fragment length polymorphism (RFLP) typing with a previously standardised method. Of the 51 RFLP types identified, 19 comprised strains of more than one serogroup. Several RFLP types included strains of five or more serogroups. To determine if sharing the same RFLP type indicates that strains are genotypically indistinguishable or merely that they are superficially similar, 31 strains were selected for further analysis with an extended range of restriction endonucleases and nucleic acid probes. In some cases, strains of a particular RFLP type were indistinguishable, while in others the restriction fragment patterns showed minor differences. It is possible that in the latter case the strains are diverging representatives of a parent clone. We conclude that analysis of restriction fragment patterns, either probed or unprobed, provides a more accurate measure of the ancestral relationship between strains than can be obtained with serological methods. |
| Databáze: | OpenAIRE |
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