A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae
| Autor: | Govindan Vijayadamodar, Loic Giot, Traci A. Mansfield, Alia Qureshi-Emili, Vaibhav A. Narayan, Pascale Pochart, Jonathan M. Rothberg, Peter Uetz, Brian C. Godwin, James R. Knight, Mark Johnston, Gerard Cagney, Diana Conover, Ying Li, Stanley Fields, Maithreyan Srinivasan, Richard S. Judson, Theodore S. Kalbfleisch, Daniel Lockshon, Meijia Yang |
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| Rok vydání: | 2000 |
| Předmět: |
Genetics
Multidisciplinary Sequence analysis Saccharomyces cerevisiae Biology Synthetic genetic array biology.organism_classification Fusion protein Protein Structure Tertiary Protein–protein interaction Fungal Proteins Open Reading Frames Protein structure Human interactome Peptide Library Two-Hybrid System Techniques Protein–protein interaction prediction Protein Binding |
| Zdroj: | Nature. 403:623-627 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/35001009 |
| Popis: | Two large-scale yeast two-hybrid screens were undertaken to identify protein-protein interactions between full-length open reading frames predicted from the Saccharomyces cerevisiae genome sequence. In one approach, we constructed a protein array of about 6,000 yeast transformants, with each transformant expressing one of the open reading frames as a fusion to an activation domain. This array was screened by a simple and automated procedure for 192 yeast proteins, with positive responses identified by their positions in the array. In a second approach, we pooled cells expressing one of about 6,000 activation domain fusions to generate a library. We used a high-throughput screening procedure to screen nearly all of the 6,000 predicted yeast proteins, expressed as Gal4 DNA-binding domain fusion proteins, against the library, and characterized positives by sequence analysis. These approaches resulted in the detection of 957 putative interactions involving 1,004 S. cerevisiae proteins. These data reveal interactions that place functionally unclassified proteins in a biological context, interactions between proteins involved in the same biological function, and interactions that link biological functions together into larger cellular processes. The results of these screens are shown here. |
| Databáze: | OpenAIRE |
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