Electrochemical stimulation of microbial cis-dichloroethene (cis-DCE) oxidation by an ethene-assimilating culture
| Autor: | Marco Zeppilli, Fabio Fava, Giulio Zanaroli, Mauro Majone, Roberta Verdini, Simona Rossetti, Federico Aulenta |
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| Přispěvatelé: | Federico Aulenta, Roberta Verdini, Marco Zeppilli, Giulio Zanaroli, Fabio Fava, Simona Rossetti, Mauro Majone |
| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
Standard hydrogen electrode Inorganic chemistry chlorinated solvent chemistry.chemical_element Bioengineering Bacillus 010501 environmental sciences Dichloroethene Electrochemistry biodegradation 01 natural sciences Oxygen environmental chemistry.chemical_compound 010608 biotechnology Molecular Biology Electrodes 0105 earth and related environmental sciences bacillus dichloroethylenes electrodes ethylenes oxidation-reduction graphite chemistry.chemical_classification oxidative biodegradation bioelectrochemical system Substrate (chemistry) General Medicine Electron acceptor Ethylenes 6. Clean water Anode Dichloroethylenes Biodegradation Environmental chemistry Degradation (geology) Graphite Oxidation-Reduction Biotechnology |
| Zdroj: | New biotechnology (Online) 30 (2013): 749–755. doi:10.1016/j.nbt.2013.04.003 info:cnr-pdr/source/autori:Aulenta F, Verdini R, Zeppilli M, Zanaroli G, Fava F, Rossetti S, Majone M/titolo:Electrochemical stimulation of microbial cis-dichloroethene (cis-DCE) oxidation by an ethene-assimilating culture/doi:10.1016%2Fj.nbt.2013.04.003/rivista:New biotechnology (Online)/anno:2013/pagina_da:749/pagina_a:755/intervallo_pagine:749–755/volume:30 New Biotechnology |
| ISSN: | 1876-4347 |
| Popis: | This study investigated the feasibility of using a polarized graphite electrode as direct or indirect (via electrolytic oxygen generation) electron acceptor to stimulate the microbial oxidation of cis-dichloroethene (cis-DCE) in contaminated groundwater. A microbial culture was enriched in the anode chamber of a bioelectrochemical cell using a mixture of cis-DCE and ethene as substrates. The bioelectrochemical cell was operated by controlling the anode potential at +1.0 V or +1.5 V versus the standard hydrogen electrode (SHE). Enhanced cometabolic removal of cis-DCE, with ethene serving as the growth substrate, was observed in batch tests with the anode polarized at +1.5 V versus SHE. At this potential, (chloro)ethenes removal was probably sustained by molecular oxygen generated at the anode from water oxidation. Conversely, negligible anaerobic degradation was observed at +1.0 V versus SHE (a potential which does not allow oxygen generation), hence suggesting that molecular oxygen is needed to initiate (chloro)ethene degradation. PCR-DGGE analysis of the microbial culture followed by band sequencing and phylogenetic analysis evidenced the selective enrichment of a Bacillus species, providing a strong indication that this microorganism was responsible for cis-DCE and ethene degradation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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