Characterization of LuWRKY36, a flax transcription factor promoting secoisolariciresinol biosynthesis in response to Fusarium oxysporum elicitors in Linum usitatissimum L. hairy roots
| Autor: | Charlène Durpoix, Eric Lainé, Cyrielle Corbin, Lucija Markulin, Samantha Drouet, Daniel Auguin, Sullivan Renouard, Christophe Hano, Charlotte Mathieu, Tatiana Lopez |
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| Přispěvatelé: | Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Institut National de la Recherche Agronomique (INRA)-Université d'Orléans (UO) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Linum Lignan Plant Science Linum usitatissimum L Models Biological Plant Roots 01 natural sciences Lignans 03 medical and health sciences chemistry.chemical_compound Abscisic acid Fusarium Plant Growth Regulators Biotic stress Stress Physiological Flax Fusarium oxysporum Genetics [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Promoter Regions Genetic Transcription factor Gene Library Plant Diseases Plant Proteins Secoisolariciresinol biology food and beverages Promoter WRKY Ethylenes biology.organism_classification WRKY protein domain Elicitor Pinoresinol-lariciresinol reductase 030104 developmental biology Biochemistry chemistry W-box Oxidoreductases Transcription Factors 010606 plant biology & botany |
| Zdroj: | Planta Planta, Springer Verlag, 2019, 250 (1), pp.347-366. ⟨10.1007/s00425-019-03172-9⟩ |
| ISSN: | 0032-0935 1432-2048 |
| DOI: | 10.1007/s00425-019-03172-9⟩ |
| Popis: | International audience; Main conclusionThe involvement of a WRKY transcription factor in the regulation of lignan biosynthesis in flax using a hairy root system is described.AbstractSecoisolariciresinol is the main flax lignan synthesized by action of LuPLR1 (pinoresinol-lariciresinol reductase 1). LuPLR1 gene promoter deletion experiments have revealed a promoter region containing W boxes potentially responsible for the response to Fusarium oxysporum. W boxes are bound by WRKY transcription factors that play a role in the response to stress. A candidate WRKY transcription factor, LuWRKY36, was isolated from both abscisic acid and Fusarium elicitor-treated flax cell cDNA libraries. This transcription factors contains two WRKY DNA-binding domains and is a homolog of AtWRKY33. Different approaches confirmed LuWRKY36 binding to a W box located in the LuPLR1 promoter occurring through a unique direct interaction mediated by its N-terminal WRKY domain. Our results propose that the positive regulator action of LuWRKY36 on the LuPLR1 gene regulation and lignan biosynthesis in response to biotic stress is positively mediated by abscisic acid and inhibited by ethylene. Additionally, we demonstrate a differential Fusarium elicitor response in susceptible and resistant flax cultivars, seen as a faster and stronger LuPLR1 gene expression response accompanied with higher secoisolariciresinol accumulation in HR of the resistant cultivar. |
| Databáze: | OpenAIRE |
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