Ratiometric Detection of Glutathione Based on Disulfide Linkage Rupture between a FRET Coumarin Donor and a Rhodamine Acceptor
Autor: | Tessa E. Steenwinkel, Shulin Wan, Rudy L. Luck, Shuai Xia, Tara Vohs, Thomas Werner, Yibin Zhang, Haiying Liu |
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Rok vydání: | 2021 |
Předmět: |
Antioxidant
Disulfide Linkage medicine.medical_treatment 010402 general chemistry 01 natural sciences Biochemistry Article Rhodamine chemistry.chemical_compound Coumarins Fluorescence Resonance Energy Transfer Tumor Cells Cultured medicine Animals Humans heterocyclic compounds Disulfides Molecular Biology Fluorescent Dyes Molecular Structure Rhodamines 010405 organic chemistry Chemistry Organic Chemistry Glutathione Acceptor Fluorescence 0104 chemical sciences Drosophila melanogaster Förster resonance energy transfer Biophysics Molecular Medicine HeLa Cells Cysteine |
Zdroj: | Chembiochem |
ISSN: | 1439-7633 1439-4227 |
Popis: | Abnormal levels of glutathione, a cellular antioxidant, can lead to a variety of diseases. We have constructed a near-infrared ratiometric fluorescent probe to detect glutathione concentrations in biological samples. The probe consists of a coumarin donor, which is connected through a disulfide-tethered linker to a rhodamine acceptor. Under the excitation of the coumarin donor at 405 nm, the probe shows weak visible fluorescence of the coumarin donor at 470 nm and strong near-infrared fluorescence of the rhodamine acceptor at 652 nm due to efficient Forster resonance energy transfer (FRET) from the donor to the acceptor. Glutathione breaks the disulfide bond through reduction, which results in a dramatic increase in coumarin fluorescence and a corresponding decrease in rhodamine fluorescence. The probe possesses excellent cell permeability, biocompatibility, and good ratiometric fluorescence responses to glutathione and cysteine with a self-calibration capability. The probe was utilized to ratiometrically visualize glutathione concentration alterations in HeLa cells and Drosophila melanogaster larvae. |
Databáze: | OpenAIRE |
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