Physiological Roles of Red Carrot Methanolic Extract and Vitamin E to Abrogate Cadmium-Induced Oxidative Challenge and Apoptosis in Rat Testes: Involvement of the Bax/Bcl-2 Ratio
| Autor: | Walaa I. E. Abdel-Badeea, Ahmed A. Mahmoud, Eman Zekry Attia, Agnieszka Najda, Abdel-Razik H. Abdel-Razik, Rabie L. Abdel Aziz, Khalaf F. Alsharif, Ibtesam S. Alanazi, Mohamed O. Mahmoud, Kamel M.A. Hassanin, Ahmed Abdel-Wahab, Usama Ramadan Abdelmohsen, Mohamed M. Abdel-Daim |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
inorganic chemicals
endocrine system Physiology medicine.medical_treatment Clinical Biochemistry Semen RM1-950 Oxidative phosphorylation Cadmium chloride medicine.disease_cause Biochemistry Article Andrology Semen quality chemistry.chemical_compound red carrot methanolic extract Bax/Bcl-2 ratio medicine Vitamin E oxidative stress Molecular Biology Chemistry food and beverages Cell Biology cadmium chloride Apoptosis Therapeutics. Pharmacology Oxidative stress Hormone |
| Zdroj: | Antioxidants, Vol 10, Iss 1653, p 1653 (2021) Antioxidants Volume 10 Issue 11 |
| ISSN: | 2076-3921 |
| DOI: | 10.3390/antiox10111653 |
| Popis: | The precise analysis of the contents of the red carrot is still ambiguous and its role in the maintenance of male fertility needs to be further reconnoitered. Hence, this study targets the physiological impacts of either red carrot methanolic extract (RCME) or vitamin E (Vit. E), co-administrated with cadmium chloride (CdCl2) on rat testes, specifically those concerned with apoptosis and oxidative challenge. Four groups of adult male rats (n = 12) are used control, CdCl2, CdCl2+Vit. E and CdCl2 + RCME. LC-MS analysis of RCME reveals the presence of 20 different phytochemical compounds. Our data clarify the deleterious effects of CdCl2 on testicular weights, semen quality, serum hormonal profile, oxidative markers and Bax/ Bcl-2 ratio. Histopathological changes in testicular, prostatic and semen vesicle glandular tissues are also observed. Interestingly, our data clearly demonstrate that co-administration of either RCME or Vit. E with CdCl2 significantly succeeded in the modulation (p < 0.05) of all of these negative effects. The most striking is that they were potent enough to modulate the Bax/Bcl-2 ratio as well as having the ability to correct the impaired semen picture, oxidant status and hormonal profile. Thus, RCME and Vit. E could be used as effective prophylactic treatments to protect the male reproductive physiology against CdCl2 insult. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|