A Novel Six-Rhodopsin System in a Single Archaeon
| Autor: | Ching Che Huang, Kang Cheng Liu, Ching-Shin Huang, Yu Cheng Lin, Rueyhung Roc Weng, Wailap Victor Ng, Yin Yu Lee, Hsiaochu Tseng, Che Wei Su, Chii Shen Yang, Hsu Yuan Fu, Yung Ning Chang |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Haloarcula marismortui
Rhodopsin genetic structures Light Archaeal Proteins Movement Gene Expression Biology medicine.disease_cause Microbiology Sensory Rhodopsins Escherichia coli Phototaxis medicine Cloning Molecular Molecular Biology Gene Expression Profiling Spectrum Analysis Biological Transport biology.organism_classification Enzymes and Proteins Recombinant Proteins Biochemistry biology.protein Bacterial rhodopsins sense organs Function (biology) Archaea |
| Zdroj: | Journal of Bacteriology. 192:5866-5873 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.00642-10 |
| Popis: | Microbial rhodopsins, a diverse group of photoactive proteins found in Archaea , Bacteria , and Eukarya , function in photosensing and photoenergy harvesting and may have been present in the resource-limited early global environment. Four different physiological functions have been identified and characterized for nearly 5,000 retinal-binding photoreceptors, these being ion transporters that transport proton or chloride and sensory rhodopsins that mediate light-attractant and/or -repellent responses. The greatest number of rhodopsins previously observed in a single archaeon had been four. Here, we report a newly discovered six-rhodopsin system in a single archaeon, Haloarcula marismortui , which shows a more diverse absorbance spectral distribution than any previously known rhodopsin system, and, for the first time, two light-driven proton transporters that respond to the same wavelength. All six rhodopsins, the greatest number ever identified in a single archaeon, were first shown to be expressed in H. marismortui , and these were then overexpressed in Escherichia coli . The proteins were purified for absorption spectra and photocycle determination, followed by measurement of ion transportation and phototaxis. The results clearly indicate the existence of a proton transporter system with two isochromatic rhodopsins and a new type of sensory rhodopsin-like transducer in H. marismortui . |
| Databáze: | OpenAIRE |
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