Isolation of human T and B lymphocytes by E-rosette gradient centrifugation. Characterization of the isolated subpopulations
| Autor: | Peter Wendelboe Hansen, S E Christiansen, Hans Erik Johnsen, M. Madsen |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Rosette Formation
Time Factors Lymphocyte Beta-Aminoethyl Isothiourea T-Lymphocytes Immunology Receptors Antigen B-Cell Cell Separation Biology Hemolysis Monocytes Antigen Phagocytosis medicine Centrifugation Density Gradient Immunology and Allergy Humans Centrifugation B cell B-Lymphocytes Monocyte T lymphocyte Molecular biology Cold Temperature medicine.anatomical_structure biology.protein Antibody beta-Aminoethyl Isothiourea |
| Zdroj: | Aalborg University |
| ISSN: | 0022-1759 |
| Popis: | Some methodological aspects of E-rosette gradient centrifugation for separation of human B and T lymphocytes are described. Treatment of sheep red blood cells (SRBC) with 2-aminoethylisothiouronium bromide (AET) accelerates and enhances E-rosette formation providing an effective and time saving method which takes less than 2 h to perform. Depletion of E-rosette forming cells (E-RFC) is almost complete, leaving less than 1% of the initial E-RFC at the interphase layer. By use of the lymphocyte donor's autologous serum containing naturally occurring anti-SRBC antibody and complement the SRBC in the rosetted T lymphocyte fraction are easily and completely lysed without damage to the lymphocytes. This procedure is shown to have little effect on the properties of the isolated T cells. The isolated lymphocyte subpopulations have been characterized by tests for lymphocyte and monocyte markers. |
| Databáze: | OpenAIRE |
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