Lysobacter silvestris sp. nov., isolated from alpine forest soil, and reclassification of Luteimonas tolerans as Lysobacter tolerans comb. nov
| Autor: | Conceição Egas, De-Chao Zhang, Rosa Margesin, Luciana Albuquerque, Hugo J.C. Froufe, Milton S. da Costa |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
DNA
Bacterial 0301 basic medicine food.ingredient Ubiquinone Luteimonas Lysobacter Forests Microbiology 03 medical and health sciences food RNA Ribosomal 16S Botany Phospholipids Phylogeny Soil Microbiology Ecology Evolution Behavior and Systematics Whole genome sequencing Base Composition Phylogenetic tree biology Strain (chemistry) Fatty Acids Nucleic Acid Hybridization Sequence Analysis DNA General Medicine Luteimonas tolerans biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques 030104 developmental biology Italy Genus Lysobacter |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 68:1571-1577 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.002710 |
| Popis: | A Gram-stain-negative, rod-shaped, motile, catalase-positive and cytochrome c oxidase-positive bacterial strain, designated AM20-91T, was isolated from alpine forest soil. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain AM20-91T was related to the genus Lysobacter and had highest 16S rRNA gene sequence similarities to the type strains of Lysobacter novalis THG-PC7T (97.8 %), Luteimonas tolerans UM1T (97.7 %) and Lysobacter ximonensis XM415T (97.0 %). The strain contained ubiquinone 8 as the predominant respiratory quinone; its polar lipid profile contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified aminophospholipids. The major cellular fatty acids (>10 %) were iso-C15 : 0, iso-C11 : 0 3-OH and iso-C11 : 0. The DNA G+C content was 63.35 % (draft genome sequence). The combined results of phylogenetic, phenotypic, DNA–DNA relatedness and chemotaxonomic analyses demonstrated that strain AM20-91T represents a novel species of the genus Lysobacter , for which the name Lysobacter silvestris sp. nov. is proposed. The type strain is AM20-91T (=DSM 104734T=LMG 30011). In this study, it is also proposed that Luteimonas tolerans be reclassified as member of the genus Lysobacter. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|