Mimicking the cardiac cycle in intact cardiomyocytes using diastolic and systolic force clamps; measuring power output
| Autor: | Bradley M. Palmer, Michiel Helmes, Niek Rijnveld, Ernst J. Breel, Aref Najafi, Jolanda van der Velden, Davide Iannuzzi |
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| Přispěvatelé: | LaserLaB - Biophotonics and Microscopy, Biophotonics and Medical Imaging, Physiology, ICaR - Heartfailure and pulmonary arterial hypertension |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Beating heart Contraction (grammar) Time Factors Physiology Systole Diastole Nanotechnology 030204 cardiovascular system & hematology Mechanotransduction Cellular Cardiomyocyte function 03 medical and health sciences 0302 clinical medicine SDG 3 - Good Health and Well-being Physiology (medical) Transducers Pressure Animals Fiber Optic Technology Myocytes Cardiac Power output Physics Feedback Physiological Cardiac Biology and Remodelling Miniaturization Cardiac cycle Signal Processing Computer-Assisted Equipment Design Original Articles Feedback loop Rats 030104 developmental biology Transducer Interferometry Force–length relation Microtechnology Cardiology and Cardiovascular Medicine Algorithms Biomedical engineering |
| Zdroj: | Cardiovascular Research Helmes, M, Najafi, A, Palmer, B M, Breel, E J, Rijnveld, N, Iannuzzi, D & van der Velden, J 2016, ' Mimicking the cardiac cycle in intact cardiomyocytes using diastolic and systolic force clamps; measuring power output ', Cardiovascular Research, vol. 111, no. 1, pp. 66-73 . https://doi.org/10.1093/cvr/cvw072 Cardiovascular Research, 111(1), 66-73. Oxford University Press Helmes, M, Najafi, A, Palmer, B M, Breel, E, Rijnveld, N, Iannuzzi, D & van der Velden, J 2016, ' Mimicking the cardiac cycle in intact cardiomyocytes using diastolic and systolic force clamps; measuring power output ', Cardiovascular Research, vol. 111, no. 1, pp. 66-73 . https://doi.org/10.1093/cvr/cvw072 |
| ISSN: | 1755-3245 0008-6363 |
| Popis: | Aims A single isolated cardiomyocyte is the smallest functional unit of the heart. Yet, all single isolated cardiomyocyte experiments have been limited by the lack of proper methods that could reproduce a physiological cardiac cycle. We aimed to investigate the contractile properties of a single cardiomyocyte that correctly mimic the cardiac cycle. Methods and results By adjusting the parameters of the feedback loop, using a suitably engineered feedback system and recording the developed force and the length of a single rat cardiomyocyte during contraction and relaxation, we were able to construct force-length relations analogous to the pressure-volume relations at the whole heart level. From the cardiac loop graphs, we obtained, for the first time, the power generated by one single cardiomyocyte. Conclusion Here, we introduce a new approach that by combining mechanics, electronics and a new type optical force transducer, can measure the force-length relationship of a single isolated cardiomyocyte undergoing a mechanical loop that mimics the pressure-volume cycle of a beating heart. |
| Databáze: | OpenAIRE |
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