A combination of (+)-catechin and (−)-epicatechin underlies the in vitro adipogenic action of Labrador tea (Rhododendron groenlandicum), an antidiabetic medicinal plant of the Eastern James Bay Cree pharmacopeia
| Autor: | Pierre S. Haddad, Meriem Ouchfoun, Louis C. Martineau, Ammar Saleem, Lina Musallam, Jose A. Guerrero-Analco, Hoda M. Eid, Michel Rapinski, Alain Cuerrier, John T. Arnason, Brendan Walshe-Roussel |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Rhododendron Labrador-tea Catechin Cell Line Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Column chromatography food Ledum Drug Discovery Animals Hypoglycemic Agents Bioassay Rhododendron groenlandicum Pharmacology Adipogenesis Plants Medicinal Traditional medicine biology Plant Extracts Chemistry Quebec Biological activity 3T3 Cells biology.organism_classification In vitro food.food 030104 developmental biology Bays 030220 oncology & carcinogenesis Medicine Traditional |
| Zdroj: | Journal of Ethnopharmacology. 178:251-257 |
| ISSN: | 0378-8741 |
| Popis: | Ethnopharmacological relevance Rhododendron groenlandicum (Oeder) Kron & Judd (Labrador tea) was identified as an antidiabetic plant through an ethnobotanical study carried out with the close collaboration of Cree nations of northern Quebec in Canada. Objectives In a previous study the plant showed glitazone-like activity in a 3T3-L1 adipogenesis bioassay. The current study sought to identify the active compounds responsible for this potential antidiabetic activity using bioassay guided fractionation based upon an in vitro assay that measures the increase of triglycerides content in 3T3-L1 adipocyte. Materials and methods Isolation and identification of the crude extract’s active constituents was carried out. The 80% ethanol extract was fractionated using silica gel column chromatography. Preparative HPLC was then used to isolate the constituents. The identity of the isolated compounds was confirmed by UV and mass spectrometry. Results Nine chemically distinct fractions were obtained and the adipogenic activity was found in fraction 5 (RGE-5). Quercetins, (+)-catechin and (−)-epicatechin were detected and isolated from this fraction. While (+)-catechin and (−)-epicatechin stimulated adipogenesis (238±26% and 187±21% relative to vehicle control respectively) at concentrations equivalent to their concentrations in the active fraction RGE-5, none afforded biological activity similar to RGE-5 or the plant’s crude extract when used alone. When cells were incubated with a mixture of the two compounds, the adipogenic activity was close to that of the crude extract (280.7±27.8 vs 311± 30%). Conclusion Results demonstrate that the mixture of (+)-catechin and (−)-epicatechin is responsible for the adipogenic activity of Labrador tea. This brings further evidence for the antidiabetic potential of R. groenlandicum and provides new opportunities to profile active principles in biological fluids or in traditional preparations. |
| Databáze: | OpenAIRE |
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