GLIAL CELLS AND COLLAGENS IN EPIRETINAL MEMBRANES ASSOCIATED WITH IDIOPATHIC MACULAR HOLES

Autor: Victor W. Renardel de Lavalette, Xiaorong Li, Roelofje J. van der Worp, Shao-Chong Bu, Roelof Kuijer, Leonoor I. Los, Johanna M. M. Hooymans, Eveline A. Huiskamp
Přispěvatelé: Restoring Organ Function by Means of Regenerative Medicine (REGENERATE)
Rok vydání: 2014
Předmět:
Male
Pathology
genetic structures
SURGERY
Fibrillar Collagens
medicine.medical_treatment
Vitrectomy
Vitreomacular traction
Basement Membrane
chemistry.chemical_compound
ULTRASTRUCTURE
VITREOMACULAR TRACTION
Gliosis
Fluorescent Antibody Technique
Indirect
Macular hole
Aged
80 and over
idiopathic macular hole
Transdifferentiation
epiretinal membrane
General Medicine
Anatomy
Middle Aged
Tissue Donors
collagens
glial cells
Female
Epiretinal membrane
Neuroglia
Myofibroblast
Tomography
Optical Coherence
Adult
FOVEA CENTRALIS
medicine.medical_specialty
MULLER CELLS
Sulfur Hexafluoride
Endotamponade
BIRDS-EYE ANALYSIS
Glial Fibrillary Acidic Protein
medicine
Humans
INTERNAL LIMITING MEMBRANE
VI COLLAGEN
Aged
HUMAN RETINA
business.industry
Colocalization
Retinal
Retinal Perforations
medicine.disease
Fibrosis
Actins
SPECIMENS
Ophthalmology
chemistry
sense organs
Carrier Proteins
business
Zdroj: Retina-The journal of retinal and vitreous diseases, 34(5), 897-906. LIPPINCOTT WILLIAMS & WILKINS
ISSN: 0275-004X
Popis: Purpose: To investigate the identity of collagens and cellular components in the epiretinal membrane (ERM) associated with full-thickness idiopathic macular hole and their clinical relevance. Methods: Pars plana vitrectomy with the peeling of internal limiting membrane and ERM was performed by 2 surgeons in 40 eyes with idiopathic macular holes. The clinical data were reviewed and the surgical specimens were processed for flat-mount and immunohistochemical analysis. Results: Epiretinal membrane is a GFAP-positive gliotic and fibrotic scar which contains newly formed Type I, III, and V collagens. Type VI collagen was not observed. Colocalization studies found cells coexpressing GFAP/CRALBP, GFAP/alpha-SMA, and alpha-SMA/CRALBP, which are consistent with transdifferentiation of Muller cells into fibroblasts and myofibroblasts. The clinically significant ERMs can be divided into two groups according to the amount of cells in ERM: sparse cellular proliferation and dense cellular proliferation. The latter group is associated with a higher chance of surgical difficulty during internal limiting membrane peeling (P = 0.006). Preoperative and postoperative visual function were not affected by the density of the cellular proliferation. Conclusion: Retinal glial cells, probably transdifferentiated Muller cells, are involved in the formation of full-thickness macular hole-associated ERMs by a gliotic and fibrotic process. Such ERMs contain newly formed Type I, III, and V collagen depositions. The cell density of ERM affects its biomechanical properties and determines the difficulty of ERM peeling.
Databáze: OpenAIRE
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