CRYOTHERAPY: A NEW TECHNIQUE TO OBTAIN GRAPEVINE PLANTS FREE OF VIRUSES
| Autor: | Ranjith Pathirana, Jean Carlos Bettoni, Murilo Dalla Costa, Aike Anneliese Kretzschmar, João Peterson Pereira Gardin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine encapsulation-dehydration medicine.medical_treatment Cryotherapy Plant Science lcsh:Plant culture Biology 01 natural sciences Cryopreservation Virus removal encapsulation-vitrification 03 medical and health sciences Time frame droplet-vitrification plant disease medicine In vitro tissue culture lcsh:SB1-1110 Vitrification Vitis Cultivar Vitis vinifera doenças de plantas business.industry encapsulamento-vitrificação food and beverages vitrificação encapsulamento-desidratação vitrification Biotechnology 030104 developmental biology business Agronomy and Crop Science 010606 plant biology & botany Food Science |
| Zdroj: | Revista Brasileira de Fruticultura v.38 n.2 2016 Revista brasileira de fruticultura Sociedade Brasileira de Fruticultura (SBF) instacron:SBFRU Revista Brasileira de Fruticultura, Vol 38, Iss 2 (2016) |
| Popis: | Through in vitro tissue culture techniques it is possible to propagate high quality nursery plants faster. Cryotherapy is a promising tool, based on in vitro culture techniques, for achieving in a short time, high frequency of regenerating plants free of viruses. The objective of this review is to present and analyze the results of research conducted in cryotherapy methods based on cryopreservation protocols for recovery of cultivars free of micro-organisms with potential agronomic interest. The main methods employed in cryotherapy are encapsulation-dehydration, vitrification, encapsulation-vitrification and droplet vitrification, which are based on the immersion of preconditioned shoot tips in liquid nitrogen, followed by their recovery in vitro on to culture media for regeneration of healthy plantlets. Improvements to cryotherapy protocols used for grapevine are still needed, since there are variations in response according to the genotype. The published research mostly relates to Vitis vinifera and the few studies applied to other species show that the protocols need to be improved. This specificity goes beyond species, with different responses among cultivars, limiting the broader application of the technology. On the other hand, traditional methods used for virus removal from infected plant materials also have limitations and therefore investment in research for the development and application of cryopreservation techniques is highly justified, considering its efficiency and low-cost, once the protocols are developed. High frequency of virus-free plants among regenerants within a short time frame is the most desirable aspect of cryotherapy. Therefore, these advantages make the technique a promising tool for institutions mandated to the development of high-health planting materials with high genetic and agronomic potential for viticulture. |
| Databáze: | OpenAIRE |
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