Far-Field Electrostatic Signatures of Macromolecular 3D Conformation
| Autor: | Gunnar Kloes, Timothy J. D. Bennett, Alma Chapet-Batlle, Ali Behjatian, Andrew J. Turberfield, Madhavi Krishnan |
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| Jazyk: | angličtina |
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| Zdroj: | Nano Letters. 22(19):7834-7840 |
| ISSN: | 1530-6992 1530-6984 |
| DOI: | 10.1021/acs.nanolett.2c02485 |
| Popis: | In solution as in vacuum, the electrostatic field distribution in the vicinity of a charged object carries information on its three-dimensional geometry. We report on an experimental study exploring the effect of molecular shape on long-range electrostatic interactions in solution. Working with DNA nanostructures carrying approximately equal amounts of total charge but each in a different three-dimensional conformation, we demonstrate that the geometry of the distribution of charge in a molecule has substantial impact on its electrical interactions. For instance, a tetrahedral structure, which is the most compact distribution of charge we tested, can create a far-field effect that is effectively identical to that of a rod-shaped molecule carrying half the amount of total structural charge. Our experiments demonstrate that escape-time electrometry (ETie/i) furnishes a rapid and facile method to screen and identify 3D conformations of charged biomolecules or molecular complexes in solution. |
| Databáze: | OpenAIRE |
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