Spelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants
| Autor: | Hilal Betul Kaya, Jan E. Leach, Zhangjun Fei, Gregory B. Martin, Andrew C. Read, Rhitu Rai, Ning Zhang, Adam J. Bogdanove, Federico Martin, Li Wang, Sara C. D. Carpenter, Matthew R. Willmann |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
lcsh:QH426-470 lcsh:Biotechnology Arabidopsis Nicotiana benthamiana Oryza sativa Computational biology Biology 01 natural sciences fluorescent tagging Prime (order theory) 03 medical and health sciences chemistry.chemical_compound prime editing lcsh:TP248.13-248.65 split GFP Genome Editing Guide RNA 030304 developmental biology 0303 health sciences Cas9 fungi food and beverages Brief Research Report biology.organism_classification plant genome editing Reverse transcriptase lcsh:Genetics chemistry Primer binding site DNA 010606 plant biology & botany |
| Zdroj: | Frontiers in Genome Editing Frontiers in Genome Editing, Vol 3 (2021) |
| ISSN: | 2673-3439 |
| DOI: | 10.3389/fgeed.2021.617553 |
| Popis: | Prime editing (PE) is a recent adaptation of the CRISPR-Cas system that uses a Cas9(H840A)-reverse transcriptase (RT) fusion and a guide RNA (pegRNA) amended with template and primer binding site (PBS) sequences to achieve RNA-templated conversion of the target DNA, allowing specified substitutions, insertions, and deletions. In the first report of PE in plants, a variety of edits in rice and wheat were described, including insertions up to 15 bp. Several studies in rice quickly followed, but none reported a larger insertion. Here, we report easy-to-use vectors for PE in dicots and monocots, their validation in Nicotiana benthamiana, rice and Arabidopsis, and an insertion of 66 bp that enabled split-GFP fluorescent tagging. |
| Databáze: | OpenAIRE |
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