Screen-printed digital microfluidics combined with surface acoustic wave nebulization for hydrogen-deuterium exchange measurements

Autor: Scott R. Heron, David R. Goodlett, J. Scott Edgar, Lucas Monkkonen, Patrick R. R. Langridge-Smith, C. Logan Mackay, Adam A. Stokes, Christophe Masselon, Daniel Winters
Přispěvatelé: Marine Geophysical Laboratory, James Cook University (JCU), Etude de la dynamique des protéomes (EDyP ), Laboratoire de Biologie à Grande Échelle (BGE - UMR S1038), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Department of Medicinal Chemistry [Seattle], University of Washington [Seattle], Laboratoire d'étude de la dynamique des protéomes (LEDyP), Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Medicinal Chemistry, University of Washington, Etude de la dynamique des protéomes [?-2019] (EDyP [?-2019]), Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Rok vydání: 2015
Předmět:
Zdroj: Monkkonen, L, Edgar, J S, Winters, D, Heron, S R, Mackay, C L, Masselon, C D, Stokes, A A, Langridge-Smith, P R R & Goodlett, D R 2016, ' Screen-printed digital microfluidics combined with surface acoustic wave nebulization for hydrogen-deuterium exchange measurements ', Journal of Chromatography A, vol. 1439, pp. 161-6 . https://doi.org/10.1016/j.chroma.2015.12.048
Journal of Chromatography A
Journal of Chromatography A, 2016, 1439, pp.161-166. ⟨10.1016/j.chroma.2015.12.048⟩
Journal of Chromatography A, Elsevier, 2016, 1439, pp.161-166. ⟨10.1016/j.chroma.2015.12.048⟩
ISSN: 1873-3778
0021-9673
DOI: 10.1016/j.chroma.2015.12.048
Popis: An inexpensive digital microfluidic (DMF) chip was fabricated by screen-printing electrodes on a sheet of polyimide. This device was manually integrated with surface acoustic wave nebulization (SAWN) MS to conduct hydrogen/deuterium exchange (HDX) of peptides. The HDX experiment was performed by DMF mixing of one aqueous droplet of angiotensin II with a second containing various concentrations of D2O. Subsequently, the degree of HDX was measured immediately by SAWN-MS. As expected for a small peptide, the isotopically resolved mass spectrum for angiotensin revealed that maximum deuterium exchange was achieved using 50% D2O. Additionally, using SAWN-MS alone, the global HDX kinetics of ubiquitin were found to be similar to published NMR data and back exchange rates for the uncooled apparatus using high inlet capillary temperatures was less than 6%.
Databáze: OpenAIRE
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