The renoprotective effects of mannitol and udenafil in renal ischemia-reperfusion injury model

Autor: Vural Kucukatay, Ali Ersin Zumrutbas, Nilay Şen Türk, Yusuf Özlülerden, Hülya Aybek, Cihan Toktaş
Rok vydání: 2017
Předmět:
creatinine blood level
medicine.medical_treatment
pyrimidine derivative
030232 urology & nephrology
Urine
Wistar rat
Kidney
lcsh:RC870-923
Nephrectomy
neutrophil gelatinase-associated lipocalin protein
rat
urea nitrogen blood level
Udenafil
chemistry.chemical_compound
0302 clinical medicine
Ngal protein
Malondialdehyde
creatinine clearance
Medicine
Mannitol
rat
animal
ligation
kidney function
Basic and Translational Research
Comet assay
Ischemia reperfusion injury
Acute-Phase Proteins/metabolism
Animals
Constriction
Diuretics
Osmotic/*pharmacology
Female
Kidney/*blood supply
Lipocalins/metabolism
Malondialdehyde/metabolism
Mannitol/*pharmacology
Phosphodiesterase 5 Inhibitors/*pharmacology
Protective Agents/pharmacology
Proto-Oncogene Proteins/metabolism
Pyrimidines/*pharmacology
Rats
Wistar
Reperfusion Injury/*prevention & control
Sulfonamides/*pharmacology
Blood urea nitrogen
Sulfonamides
acute phase protein
malonaldehyde
osmotic diuretic agent
reperfusion injury
Diuretics
Osmotic
Lipocalins
female
Udenafil this
oncoprotein
Reperfusion Injury
030220 oncology & carcinogenesis
histopathology
Original Article
lipocalin
medicine.drug
medicine.medical_specialty
Urology
Protective Agents
Article
animal tissue
lipid peroxidation assay
03 medical and health sciences
Lipocalin-2
vascularization
comet assay
Proto-Oncogene Proteins
udenafil
sulfonamide
spectrophotometry
controlled study
protective agent
Creatinine
nonhuman
business.industry
animal model
kidney ischemia
Phosphodiesterase 5 Inhibitors
lcsh:Diseases of the genitourinary system. Urology
Pyrimidines
chemistry
DNA damage
phosphodiesterase V inhibitor
business
experimental kidney disease
metabolism
neutrophil gelatinase associated lipocalin
urine sampling
Acute-Phase Proteins
Zdroj: Investigative and Clinical Urology, Vol 58, Iss 4, Pp 289-295 (2017)
Investigative and Clinical Urology
ISSN: 2466-054X
2466-0493
DOI: 10.4111/icu.2017.58.4.289
Popis: Purpose: The aim of this study was to investigate and compare the effects of udenafil and mannitol in an experimental renal ischemia-reperfusion (I/R) injury model.
Materials and Methods: A total of 64 female Wister Albino rats were used. Right nephrectomy was performed in all groups. In the control group; I/R injury was not performed. In the I/R group; left renal pedicle was clamped for 45 minutes and then underwent 60 minutes and 24 hours of reperfusion. In the mannitol group; 1 mL 20% mannitol was given intravenously 15 minutes before clamping. In the udenafil group; 10-mg/kg udenafil was given orally 1 hour before clamping. Creatinine (Cr), blood urea nitrogen (BUN), Cr clearance, malondialdehyde, neutrophil gelatinase associated lipocalin (NGAL), histological examination and DNA damage (Comet Assay method) levels were compared in tissue, serum and urine samples.
Results: Udenafil had a better protective effect than mannitol according to biochemical parameters (Cr, BUN, Cr clearance, and NGAL levels) and histopathological findings when compared with the I/R group. In the Comet sampling analysis no significant difference was detected.
Conclusions: Udenafil has a better renoprotective effect than mannitol against I/R injury and this effect supports more functional improvements. Further clinical trials are needed to demonstrate those effects and clinical utility of udenafil for that purpose in humans.
Databáze: OpenAIRE
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