The C-Terminal Fragment of Prostate-Specific Antigen, a 2331 Da Peptide, as a New Urinary Pathognomonic Biomarker Candidate for Diagnosing Prostate Cancer
| Autor: | Junko Oosaga, Kenji Nakayama, Shinichi Iwamoto, Kuniko Ikawa, Shin-ichiro Kawabata, Hiroaki Tsuji, Sadanori Sekiya, Osamu Ogawa, Naoki Terada, Koichi Tanaka, Takahiro Inoue, Takayuki Goto, Yu Miyazaki, Shigeki Kajihara |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Proteomics Aging Pathology lcsh:Medicine Urine urologic and male genital diseases Biochemistry Mass Spectrometry Analytical Chemistry Prostate cancer Spectrum Analysis Techniques Sequence Analysis Protein Pathognomonic Medicine and Health Sciences lcsh:Science Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Digital Rectal Examination Aged 80 and over Clinical Chemistry Multidisciplinary Prostate Cancer Middle Aged Body Fluids Chemistry Prostate-specific antigen Bioassays and Physiological Analysis Oncology Physical Sciences Biomarker (medicine) Anatomy Research Article PCA3 medicine.medical_specialty Urology Urinary system Research and Analysis Methods Antigen Diagnostic Medicine Biomarkers Tumor medicine Humans Amino Acid Sequence Mass screening Aged business.industry lcsh:R Prostatic Neoplasms Biology and Life Sciences Cancers and Neoplasms Prostate-Specific Antigen medicine.disease Peptide Fragments Genitourinary Tract Tumors Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Cancer research lcsh:Q Clinical Medicine Biochemical Analysis business Biomarkers |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 9, p e107234 (2014) |
| ISSN: | 1932-6203 |
| Popis: | BACKGROUND AND OBJECTIVES: Prostate cancer (PCa) is one of the most common cancers and leading cause of cancer-related deaths in men. Mass screening has been carried out since the 1990s using prostate-specific antigen (PSA) levels in the serum as a PCa biomarker. However, although PSA is an excellent organ-specific marker, it is not a cancer-specific marker. Therefore, the aim of this study was to discover new biomarkers for the diagnosis of PCa. MATERIALS AND METHODS: We focused on urine samples voided following prostate massage (digital rectal examination [DRE]) and conducted a peptidomic analysis of these samples using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS(n)). Urinary biomaterials were concentrated and desalted using CM-Sepharose prior to the following analyses being performed by MALDI-TOF/MS(n): 1) differential analyses of mass spectra; 2) determination of amino acid sequences; and 3) quantitative analyses using a stable isotope-labeled internal standard. RESULTS: Multivariate analysis of the MALDI-TOF/MS mass spectra of urinary extracts revealed a 2331 Da peptide in urine samples following DRE. This peptide was identified as a C-terminal PSA fragment composed of 19 amino acid residues. Moreover, quantitative analysis of the relationship between isotope-labeled synthetic and intact peptides using MALDI-TOF/MS revealed that this peptide may be a new pathognomonic biomarker candidate that can differentiate PCa patients from non-cancer subjects. CONCLUSION: The results of the present study indicate that the 2331 Da peptide fragment of PSA may become a new pathognomonic biomarker for the diagnosis of PCa. A further large-scale investigation is currently underway to assess the possibility of using this peptide in the early detection of PCa. |
| Databáze: | OpenAIRE |
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