Molecular Basis for Different Levels of tet(M) Expression in Streptococcus pneumoniae Clinical Isolates
| Autor: | Laurent Gutmann, Sophie Grondin, Arnaud Firon, Claire Poyart, Emmanuelle Varon, Isabelle Podglajen, Patrick Grohs, Patrick Trieu-Cuot |
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| Přispěvatelé: | Service de microbiologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Biologie des Bactéries pathogènes à Gram-positif, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Centre de Référence du Pneumocoque, Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service de Bactériologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), This study was supported by a subvention of the Institut de Veille Sanitaire to the CNRP and by an INSERM grant., Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP)-Hôpital Européen Georges Pompidou [APHP] ( HEGP ), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Hôpital Européen Georges Pompidou [APHP] ( HEGP ), Institut Cochin ( UM3 (UMR 8104 / U1016) ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Cochin [AP-HP], Centre de Recherche des Cordeliers ( CRC (UMR_S 872) ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
MESH: Tetracycline Resistance
MESH: Acinetobacter/drug effects Mutant MESH: Burkholderia/ultrastructure MESH: Pseudomonas/drug effects Oligosaccharides [ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: Burkholderia/metabolism medicine.disease_cause Microscopy Atomic Force Glucuronic Acid MESH: Anti-Bacterial Agents/pharmacology MESH: Acinetobacter/metabolism MESH: Microscopy Confocal Pharmacology (medical) MESH : Biofilms/drug effects MESH: Microscopy Atomic Force 0303 health sciences Microscopy Confocal biology Acinetobacter Hexuronic Acids Tetracycline Resistance MESH : Hexuronic Acids/chemistry 3. Good health Anti-Bacterial Agents MESH : Acinetobacter/ultrastructure Infectious Diseases Streptococcus pneumoniae MESH: Acinetobacter/ultrastructure MESH : Anti-Bacterial Agents/pharmacology MESH : Glucuronic Acid/chemistry MESH : Burkholderia/ultrastructure MESH: Bacterial Proteins/metabolism MESH: Alginates/chemistry medicine.drug Tetracycline Alginates Burkholderia MESH: Hexuronic Acids/chemistry Enterococcus faecalis Microbiology Frameshift mutation MESH: Glucuronic Acid/chemistry 03 medical and health sciences MESH : Streptococcus pneumoniae/metabolism Bacterial Proteins Mechanisms of Resistance Pseudomonas MESH : Burkholderia/drug effects medicine MESH : Microscopy Confocal Gene 030304 developmental biology MESH: Biofilms/drug effects Pharmacology MESH : Acinetobacter/metabolism Reporter gene MESH : Tetracycline Resistance MESH: Burkholderia/drug effects 030306 microbiology MESH: Oligosaccharides/chemical synthesis Promoter MESH: Streptococcus pneumoniae/metabolism MESH : Burkholderia/metabolism biochemical phenomena metabolism and nutrition biology.organism_classification Molecular biology [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH : Microscopy Atomic Force MESH : Pseudomonas/drug effects MESH : Acinetobacter/drug effects MESH : Bacterial Proteins/metabolism Biofilms MESH : Oligosaccharides/chemical synthesis MESH : Alginates/chemistry |
| Zdroj: | Antimicrobial Agents and Chemotherapy Antimicrobial Agents and Chemotherapy, 2012, 56 (10), pp.5040-5045. ⟨10.1128/aac.00939-12⟩ Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 2012, 56 (10), pp.5040-5045. ⟨10.1128/aac.00939-12⟩ Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 2012, 56 (10), pp.5040-5045. 〈10.1128/aac.00939-12〉 |
| ISSN: | 0066-4804 1098-6596 |
| DOI: | 10.1128/aac.00939-12⟩ |
| Popis: | Seventy-four unrelated clinical isolates of Streptococcus pneumoniae harboring the tet (M) gene were studied. Seven strains with low tetracycline (Tc) MICs (0.25 to 0.5 μg/ml) were found to harbor truncated tet (M) alleles that were inactivated by different frameshift mutations. In contrast, five strains bore deletions in the tet (M) promoter region, among which four displayed increased Tc MICs (16 to 64 μg/ml). The same promoter mutations were detected in Tc-resistant mutants selected in vitro from various susceptible strains. Sequence analysis revealed that these deletions might impede the formation of the transcriptional attenuator located immediately upstream of tet (M). Expression in Enterococcus faecalis of a tet (M) reporter gene transcribed from these promoter mutants conferred a level of Tc resistance similar to that observed in the parental S. pneumoniae strains. These results show that different levels of Tc susceptibility found in clinical isolates of S. pneumoniae can be explained by frameshift mutations within tet (M) and by alterations of the upstream transcriptional attenuator. |
| Databáze: | OpenAIRE |
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