Effect of taurine intervention on oleic acid-induced primary hepatocyte steatosis in orange-spotted grouper (Epinephelus coioides)
| Autor: | Fakai Bai, Hanmo Feng, Ruyi Xiao, Ji-Dan Ye, Xingjian Niu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
differential expression gene
medicine.medical_specialty Taurine Orange-spotted grouper QH301-705.5 Peroxisome proliferator-activated receptor hepatocyte steatosis General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Internal medicine medicine Grouper Biology (General) chemistry.chemical_classification Fatty acid metabolism biology Lipid metabolism medicine.disease biology.organism_classification Endocrinology chemistry rna-seq Fatty acid elongation Steatosis taurine General Agricultural and Biological Sciences epinephelus coioides |
| Zdroj: | Archives of Biological Sciences, Vol 73, Iss 4, Pp 491-501 (2021) |
| ISSN: | 1821-4339 0354-4664 |
| DOI: | 10.2298/abs210916043x |
| Popis: | Examination of the molecular mechanism of taurine regulation of lipid metabolism in fish is limited. In this study, an oleic acid (OA)-induced hepatocyte steatosis model of orange-spotted grouper (Epinephelus coioides) was established for the first time. The model was used to test the effect of taurine on steatosis hepatocytes in Control, High-fat (0.4 mM OA) and Taurine (0.4 mM OA + 2 mM taurine) experimental groups of fish. Hepatocyte samples were subjected to transcriptome analysis. A total of 99634 unigenes was assembled, 69982 unigenes were annotated and 1831 differentially expressed genes (DEGs) in Control vs High-fat group, and 526 DEGs in the High-fat vs Taurine group were identified, of which 824 DEGs (Control vs High-fat) and 237 DEGs (High-fat vs Taurine) were observed to be upregulated, and 1007 DEGs (Control vs High-fat) and 289 DEGs (High-fat vs Taurine) were downregulated after taurine intervention. These genes are involved in peroxisome proliferator-activated receptor (PPAR) and 5' AMP-activated protein kinase (AMPK) signaling pathways, fatty acid elongation, primary bile acid biosynthesis, glycerophospholipid and glycerolipid metabolism. The findings provide new clues in understanding the regulatory role of taurine in lipid and fatty acid metabolism of fish. It is hoped that the obtained results will help in the design of feed formulations to improve grouper growth from the perspective of aquaculture nutrition. |
| Databáze: | OpenAIRE |
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