Therapeutic Role of Bone Marrow-Derived Mesenchymal Stem Cells in Controlling Prognosis of Hepatocellular Carcinoma in a Murine Model
| Autor: | Wafaa, Mansour, Manal, Kamel, Emad, Elzayat, Shimaa, Atta, Dalia, Mahmood, Hanaa, Abd El Fattah El Sayed, Taghreed, Hussein, Sara, Saber |
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| Rok vydání: | 2022 |
| Předmět: |
Interleukin 2
Carcinoma Hepatocellular Mesenchymal Stem Cell Transplantation Mice Bone Marrow Interferon Animals Humans Medicine Diethylnitrosamine Transplantation business.industry Liver Neoplasms Mesenchymal stem cell Cancer Mesenchymal Stem Cells Prognosis medicine.disease Liver regeneration Rats Disease Models Animal Treatment Outcome medicine.anatomical_structure Liver Hepatocellular carcinoma Cancer research Bone marrow Liver function business medicine.drug |
| Zdroj: | Experimental and Clinical Transplantation. 20:62-68 |
| ISSN: | 2146-8427 1304-0855 |
| DOI: | 10.6002/ect.2020.0495 |
| Popis: | Objectives Hepatocellular carcinoma is the fourth leading cause of cancer deaths in the world. Conventional methods of cancer therapy are either invasive or have undesirable side effects. Therefore, exploring new therapeutic strategies to control the progression of hepatocellular carcinoma, such as cell-based therapies, is a key issue for prolonging patient survival. In this study, we aimed to evaluate tumor suppressive effects of mesenchymal stem cells on the in vivo progression of hepatocellular carcinoma in murine model. Materials and methods Hepatocellular carcinoma was induced in 40 rats with diethylnitrosamine. Rats were divided into 4 groups: 1 group injected with diethylnitrosamine only, 1 group injected with diethylnitrosamine and 1 dose of rat bone marrowderived mesenchymal stem cells, 1 group injected with diethylnitrosamine and 2 doses of rat bone marrowderived mesenchymal stem cells, and 1 group was injected with diethylnitrosamine and 3 doses of rat bone marrow-derived mesenchymal stem cells. Rats were killed after 1 month of dose 3. Liver specimens were histopathologically examined, and serum samples were examined for liver function and cytokines. Results Histopathological examination revealed that mesenchymal stem cell transplant induced liver regeneration. It also improved liver function as revealed by decreased levels of alanine and aspartate aminotransferase. Mesenchymal stem cells also repaired the immunopathology of the liver environment, as it decreased levels of interleukin 2 and 10, tumor necrosis factor α, and interferon γ. Conclusions Mesenchymal stem cell infusion significantly enhanced hepatic structure and function of livers in a rat hepatocellular carcinoma model. |
| Databáze: | OpenAIRE |
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