Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells on Concanavalin A-Induced Acute Liver Injury in Mice
| Autor: | Sanae Fujinaka, Yumi Hayashi, Yoshinobu Baba, Tetsuya Ishikawa, Yoko Tsutsui, Kenta Nakagiri, Shingo Nakagawa, Ayano Kanou, Ryoji Iwaki, Hiroshi Yukawa, Tatsuya Yamada, Yusuke Ogihara, Yasuma Yoshizumi, Masatoshi Ishigami, Yuki Kanou, Tomomi Ohara |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
endocrine system animal diseases Adipose tissue chemical and pharmacologic phenomena Article Cell therapy 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Medicine General Environmental Science biology Cluster of differentiation business.industry hemic and immune systems eye diseases Transplantation 030104 developmental biology chemistry Concanavalin A 030220 oncology & carcinogenesis Ionomycin biology.protein Cancer research General Earth and Planetary Sciences Stem cell business |
| Zdroj: | Cell Medicine. 9:21-33 |
| ISSN: | 2155-1790 |
| Popis: | Cell therapy with adipose tissue-derived stem cells (ASCs) is expected to be a candidate for the treatment of fulminant hepatic failure (FHF), which is caused by excessive immune responses. In order to evaluate the therapeutic effects of ASCs on FHF, the in vitro and in vivo immunomodulatory effects of ASCs were examined in detail in the mouse model. The in vitro effects of ASCs were examined by assessing their influence on the proliferation of lymphomononuclear cells (LMCs) stimulated with three kinds of mitogens: phorbol 12-myristate 13-acetate (PMA) plus ionomycin, concanavalin A (ConA), and lipopolysaccharide (LPS). The proliferation of LMCs was efficiently suppressed in a dose-dependent manner by ASCs in the cases of PMA plus ionomycin stimulation and ConA stimulation, but not in the case of LPS stimulation. The in vivo effects of transplanted ASCs were examined in the murine FHF model induced by ConA administration. The ALT levels and histological inflammatory changes in the ConA-administered mice were apparently relieved by the transplantation of ASCs. The analysis of mRNA expression patterns in the livers indicated that the expressions of the cytokines such as Il-6, Il-10, Ifn-γ, and Tnf-α, and the cell surface markers such as Cd3γ, Cd4, Cd8α, Cd11b, and Cd11c were downregulated in the ASC-transplanted mice. The immunomodulatory and therapeutic effects of ASCs were confirmed in the mouse model both in vitro and in vivo. These suggest that the cell therapy with ASCs is beneficial for the treatment of FHF. |
| Databáze: | OpenAIRE |
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