An α-Linolenic Acid-Rich Formula Reduces Oxidative Stress and Inflammation by Regulating NF-κB in Rats with TNBS-Induced Colitis
| Autor: | Mohamed Skiba, Frédéric Ziegler, Guillaume Savoye, Jean-Michel Chardigny, Ayman Ibrahim, Moïse Coëffier, Pierre Déchelotte, Khaly Mbodji, Aktham Hassan, Frédéric Bounoure, Rachel Marion-Letellier |
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| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Erythrocytes Colon Nitric Oxide Synthase Type II Medicine (miscellaneous) Dinoprost medicine.disease_cause Nitric oxide Rats Sprague-Dawley chemistry.chemical_compound Internal medicine medicine Animals Chymotrypsin Colitis Unsaturated fatty acid Nutrition and Dietetics alpha-Linolenic acid Fatty Acids NF-kappa B alpha-Linolenic Acid Glutathione medicine.disease Eicosapentaenoic acid Diet Rats Oxidative Stress Endocrinology Trinitrobenzenesulfonic Acid chemistry Biochemistry Cytokines Eicosanoids Interferons Docosapentaenoic acid Oxidative stress |
| Zdroj: | The Journal of Nutrition. 140:1714-1721 |
| ISSN: | 0022-3166 |
| DOI: | 10.3945/jn.109.119768 |
| Popis: | We have previously shown that α-linolenic acid (ALA), a (n-3) PUFA exerts in vitro antiinflammatory effects in the intestine. In this study, we aimed to evaluate its effect on inflammatory and oxidative stress in a colitis model. Colitis was induced in 2 groups at d 0 by intrarectal injection of 2-4-6-trinitrobenzen sulfonic acid (TNBS), whereas the control group received the vehicle. Rats we fed 450 mg . kg(-1) . d(-1) of ALA (TNBS+ALA) while the other colitic group (TNBS) and the control group were fed an isocaloric corn oil formula for 14 d (from d -7 to d 7). RBC fatty acid composition was assessed. Oxidative stress was studied by measuring urinary 8-isoprostanes (8-IP) and colon glutathione (GSH) concentration and inducible nitric oxide synthase (iNOS) expression. Colitis was assessed histologically, by production of proinflammatory mediators, including cytokines, leukotrienes B(4) (LTB(4)), and cyclooxygenase-2 (COX-2) and by nuclear factor-κB (NF-κB) activation. The ALA-rich diet significantly increased the RBC levels of ALA, eicosapentaenoic acid, and docosapentaenoic acid (n-3) compared with the TNBS group (P < 0.01 for all). The beneficial effect of ALA supplementation on oxidative stress was reflected by lower urinary 8-IP levels (P < 0.05), a normalized colon GSH concentration (P < 0.01), and reduced colon iNOS expression (P < 0.05) compared with the TNBS group. ALA also protected against colon inflammation as assessed by lower tumor necrosis factor-α secretion and mRNA level (P < 0.05), reduced NF-κB activation (P = 0.01), and lower colon lipid mediator concentrations such as LTB(4) and COX-2 (P < 0.05) compared with the TNBS group. These findings show that an ALA-rich formula is beneficial to TNBS-induced colitic rats via inhibition of oxidative and inflammatory stress. |
| Databáze: | OpenAIRE |
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