Aberrant DNA methylation ofE-cadherin andp16 genes in rat lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine
| Autor: | Hiromasa Fujii, Ayumi Kato, Yozo Shimoichi, Kyoko Shimizu, Kanya Honoki, Toshifumi Tsujiuchi |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Cancer Research Lung Neoplasms Nitrosamines Bisulfite sequencing Adenocarcinoma Biology medicine.disease_cause chemistry.chemical_compound Reference Values Gene expression medicine Animals Rats Wistar Lung Molecular Biology Gene Reverse Transcriptase Polymerase Chain Reaction Cadherin Genes p16 DNA Methylation Cadherins Molecular biology Reverse transcriptase Rats Gene Expression Regulation Neoplastic chemistry DNA methylation Cancer research Carcinogenesis DNA |
| Zdroj: | Molecular Carcinogenesis. 45:106-111 |
| ISSN: | 1098-2744 0899-1987 |
| DOI: | 10.1002/mc.20162 |
| Popis: | The study aimed to assess the involvement of aberrant DNA methylation in lung carcinogenesis by measuring expressions of E-cadherin and p16 genes, and their DNA methyation status in the 5′ upstream region in rat lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine (BHP). Six-week-old male Wistar rats were given 2000 ppm BHP in their drinking water for 12 wk, and maintained without further treatment until they were sacrificed at 25 wk. A total of nine lung adenocarcinomas were collected, and total RNA from each sample was extracted for assessment of gene expression by real-time reverse transcription (RT)-polymerase chain reaction (PCR). Expressions of E-cadherin and p16 genes were significantly reduced in lung adenocarcinomas (P |
| Databáze: | OpenAIRE |
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