Transcriptional regulation of the salivary histatin gene: finding of a strong positive regulatory element and its binding protein
| Autor: | Ken Ouryouji, Hiroo Miyazawa, Shigeru Yanagisawa, Yoshihisa Fujigaki, Yasuhiro Imamura, Yuriko Oomori, Pao-Li Wang |
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| Rok vydání: | 2008 |
| Předmět: |
Transcription
Genetic Gene prediction Molecular Sequence Data Histatins Biology Biochemistry Mice stomatognathic system Transcription (biology) Gene expression Chlorocebus aethiops medicine Transcriptional regulation Animals Humans Cloning Molecular Promoter Regions Genetic Saliva Molecular Biology Gene Sequence Deletion Salivary gland Base Sequence Nucleotides Gene Expression Profiling Nuclear Proteins General Medicine Molecular biology Molecular Weight medicine.anatomical_structure Gene Expression Regulation Regulatory sequence Organ Specificity Histatin COS Cells NIH 3T3 Cells HeLa Cells Protein Binding |
| Zdroj: | Journal of biochemistry. 145(3) |
| ISSN: | 1756-2651 |
| Popis: | Histatins are salivary proteins found and expressed in human salivary glands. They play a role in the non-immune system of antimicrobial defense, for instance, against Candida albicans. The transcriptional regulatory sequences of the histatin gene, HIS1, have remained obscure for a long time. Here, we cloned the putative promoter from human genomic DNA and tested it in a luciferase reporter system. This promoter is much more active in salivary gland cells than in other cell types. Analysis of deletion mutants revealed that the region encompassing -2254 to -1748 is a strong positive transcriptional element, and its functional core sequence (termed HTN27 box) works in correct and reverse orientations in synergy with downstream sequences, the region spanning -680 to +28 and a proximal promoter. The plus single-stranded HTN27 box is specifically bound by a 100 kDa protein that is present in HSG cells, but not in HeLa cells. These findings indicate that the regulation of the histatin gene expression may be intricate, and it seems to have a cell-type preference in the salivary gland cells. |
| Databáze: | OpenAIRE |
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