Characterization of four virulent Klebsiella pneumoniae bacteriophages, and evaluation of their potential use in complex phage preparation
| Autor: | Evgeniy Zhilenkov, Fedor Zurabov |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Klebsiella Phage cocktails Phage therapy Glycoside Hydrolases medicine.medical_treatment viruses 030106 microbiology Virulence Virus Attachment ESKAPE Myoviridae Genome Viral Host Specificity lcsh:Infectious and parasitic diseases Siphoviridae 03 medical and health sciences Viral Proteins Antibiotic resistance Bacteriolysis Virology Lysogenic cycle medicine Caudovirales Bacteriophages lcsh:RC109-216 biology Research Temperature Hydrogen-Ion Concentration biology.organism_classification Depolymerases Klebsiella Infections Virus Latency Klebsiella pneumoniae 030104 developmental biology Infectious Diseases Lytic cycle Biofilms DNA Viral |
| Zdroj: | Virology Journal, Vol 18, Iss 1, Pp 1-20 (2021) Virology Journal |
| Popis: | BackgroundNowadays, hundreds of thousands of deaths per year are caused by antibiotic resistant nosocomial infections and the prognosis for future years is much worse, as evidenced by modern research. Bacteria of theKlebsiellagenus are one of the main pathogens that cause nosocomial infections. Among the many antimicrobials offered to replace or supplement traditional antibiotics, bacteriophages are promising candidates.MethodsThis article presents microbiological, physicochemical and genomic characterization of 4 virulent bacteriophages belonging toSiphoviridae,MyoviridaeandPodoviridaefamilies. Phages were studied by electron microscopy; their host range, lytic activity, adsorption rate, burst size, latent period, frequency of phage-resistant forms generation, lysis dynamics and sensitivity of phage particles to temperature and pH were identified; genomes of all 4 bacteriophages were studied by restriction digestion and complete genome sequence.ResultsStudied phages showed wide host range and high stability at different temperature and pH values. In contrast with single phages, a cocktail of bacteriophages lysed all studied bacterial strains, moreover, no cases of the emergence of phage-resistant bacterial colonies were detected. Genomic data proved that isolated viruses do not carry antibiotic resistance, virulence or lysogenic genes. Three out of four bacteriophages encode polysaccharide depolymerases, which are involved in the degradation of biofilms and capsules.ConclusionsThe bacteriophages studied in this work are promising for further in vivo studies and might be used in phage therapy as part of a complex therapeutic and prophylactic phage preparation. The conducted studies showed that the complex preparation is more effective than individual phages. The use of the complex phage cocktail allows to extend the lytic spectrum, and significantly reduces the possibility of phage-resistant forms generation. |
| Databáze: | OpenAIRE |
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