Antiseizure potential of peptides from the venom of social wasp Chartergellus communis against chemically-induced seizures
| Autor: | Maria Varela Torres Quintanilha, Márcia Renata Mortari, Adolfo Carlos Barros de Souza, Fernando Zamudio-Zuñiga, Kamila Soares Lopes, Lourival D. Possani |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Wasps Context (language use) Venom Wasp Venoms Pharmacology Toxicology 01 natural sciences 03 medical and health sciences Epilepsy Pharmacotherapy Seizures Medicine Animals Adverse effect ED50 0303 health sciences business.industry 010604 marine biology & hydrobiology 030302 biochemistry & molecular biology medicine.disease Motor coordination Disease Models Animal Pilocarpine Pentylenetetrazole Anticonvulsants business Peptides medicine.drug |
| Zdroj: | Toxicon : official journal of the International Society on Toxinology. 194 |
| ISSN: | 1879-3150 |
| Popis: | Epilepsy is one of the most common neurological diseases in the world. The objective of this research was to investigate a new peptide from the venom of the social wasp Chartergellus communis useful to the study or pharmacotherapy of epilepsy. The wasps were collected, and their venom was extracted. Afterward, the steps of fractionation, sequencing, and identification were carried out to obtain four peptides. These molecules were synthesized for behavioral evaluation tests and electroencephalographic assays to determine their antiseizure potential (induction of acute seizures using the chemical compounds, pentylenetetrazole - PTZ, and pilocarpine - PILO) and analysis of neuropharmacological profile (general spontaneous activity and alteration in motor coordination). Chartergellus-CP1 (i.c.v. - 3.0 μg/animal) caused beneficial alterations in some of the parameters evaluated in both models: PTZ (latency and duration of maximum seizures) and PILO (latency and duration of, and protection against, maximum seizures, and reduction of the median of the seizure scores. When evaluated in 3 doses in the seizure model induced by PILO, the dose of 3.0 μg/animal protected the animals against seizures, with an estimated ED50 of 1.49 μg/animal. Electroencephalographic evaluation of Chartergellus-CP1 showed an improvement in latency, quantity, and percentage of protection against generalized electroencephalographic seizures in the PILO model. Further, Chartergellus-CP1 did not cause adverse effects on general spontaneous activity and motor coordination of animals. This study demonstrated how compounds isolated from wasps' venom may be important resources in the search for new drugs. Such compounds can be considered valuable therapeutic and biotechnological tools for the study and future treatment of epileptic disorders. In this context, a peptide that is potentially useful for epilepsy pharmacotherapy was identified in the venom of C. communis. |
| Databáze: | OpenAIRE |
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