AA16, a new lytic polysaccharide monooxygenase family identified in fungal secretomes

Autor: David Navarro, Senta Heiss-Blanquet, Sacha Grisel, Antoine Margeot, Camille Filiatrault-Chastel, Mathieu Fanuel, Mireille Haon, Bernard Henrissat, Didier Chevret, Jean-Guy Berrin, Isabelle Herpoël-Gimbert
Přispěvatelé: Biodiversité et Biotechnologie Fongiques (BBF), École Centrale de Marseille (ECM)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA), IFP Energies nouvelles (IFPEN), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Architecture et fonction des macromolécules biologiques (AFMB), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0106 biological sciences
Filamentous fungi
activité cellulolytique
LPMO Biofuels
lcsh:Biotechnology
Lignocellulosic biomass
Biotechnologies
Management
Monitoring
Policy and Law
01 natural sciences
7. Clean energy
Applied Microbiology and Biotechnology
lcsh:Fuel
Pichia pastoris
Cell wall
03 medical and health sciences
lcsh:TP315-360
010608 biotechnology
lcsh:TP248.13-248.65
Cellulolytic enzymes production
LPMO
production d'enzyme
Trichoderma reesei
biomasse végétale
030304 developmental biology
0303 health sciences
Aspergillus
Plant biomass
biology
Renewable Energy
Sustainability and the Environment
Research
Aspergillus aculeatus
Aspergillus niger
food and beverages
[CHIM.CATA]Chemical Sciences/Catalysis
Secretomics
15. Life on land
biology.organism_classification
champignon filamenteux
Biofuels
General Energy
Biochemistry
biocarburant
[SDE]Environmental Sciences
Biotechnology
Zdroj: Biotechnology for Biofuels, Vol 12, Iss 1, Pp 1-15 (2019)
Biotechnology for Biofuels
Biotechnology for Biofuels, BioMed Central, 2019, 12 (1), ⟨10.1186/s13068-019-1394-y⟩
Biotechnology for Biofuels (12), . (2019)
Biotechnology for Biofuels, 2019, 12 (1), ⟨10.1186/s13068-019-1394-y⟩
ISSN: 1754-6834
DOI: 10.1186/s13068-019-1394-y
Popis: Background Lignocellulosic biomass is considered as a promising alternative to fossil resources for the production of fuels, materials and chemicals. Efficient enzymatic systems are needed to degrade the plant cell wall and overcome its recalcitrance. A widely used producer of cellulolytic cocktails is the ascomycete Trichoderma reesei, but this organism secretes a limited set of enzymes. To improve the saccharification yields, one strategy is to upgrade the T. reesei enzyme cocktail with enzymes produced by other biomass-degrading filamentous fungi isolated from biodiversity. Results In this study, the enzymatic cocktails secreted by five strains from the genus Aspergillus (Aspergillus japonicus strains BRFM 405, 1487, 1489, 1490 and Aspergillus niger strain BRFM 430) were tested for their ability to boost a T. reesei reference cocktail for the saccharification of pretreated biomass. Proteomic analysis of fungal secretomes that significantly improved biomass degradation showed that the presence of proteins belonging to a putative LPMO family previously identified by genome analysis and awaiting experimental demonstration of activity. Members of this novel LPMO family, named AA16, are encountered in fungi and oomycetes with life styles oriented toward interactions with plant biomass. One AA16 protein from Aspergillus aculeatus (AaAA16) was produced to high level in Pichia pastoris. LPMO-type enzyme activity was demonstrated on cellulose with oxidative cleavage at the C1 position of the glucose unit. AaAA16 LPMO was found to significantly improve the activity of T. reesei CBHI on cellulosic substrates. Conclusions Although Aspergillus spp. has been investigated for decades for their CAZymes diversity, we identified members of a new fungal LPMO family using secretomics and functional assays. Properties of the founding member of the AA16 family characterized herein could be of interest for use in biorefineries. Electronic supplementary material The online version of this article (10.1186/s13068-019-1394-y) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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