Extending In-Plane Impedance Measurements from 2D to 3D Cultures: Design Considerations
| Autor: | Zay Yar Oo, Sally L. McArthur, Lana Cleuren, Sorel E. De Leon, Paul R. Stoddart |
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| Přispěvatelé: | McArthur, Sally L/0000-0002-4914-6989, Stoddart, Paul/0000-0003-4882-9400, De Leon, Sorel/0000-0002-0227-2910, De Leon, Sorel E., Cleuren , Lana, Oo, Zay Yar, Stoddart, Paul R., McArthur, Sally L. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Bioengineering 3d model 02 engineering and technology lcsh:Technology Signal Article 03 medical and health sciences 3D cell culture tissue culture lcsh:QH301-705.5 Electrical impedance 030304 developmental biology 0303 health sciences in situ monitoring lcsh:T 021001 nanoscience & nanotechnology Dielectric spectroscopy electrical impedance spectroscopy In plane cell proliferation lcsh:Biology (General) Electrode 0210 nano-technology Biomedical engineering Protein adsorption |
| Zdroj: | Bioengineering Volume 8 Issue 1 Bioengineering, Vol 8, Iss 11, p 11 (2021) |
| ISSN: | 2306-5354 |
| DOI: | 10.3390/bioengineering8010011 |
| Popis: | Three-dimensional (3D) cell cultures have recently emerged as tools for biologically modelling the human body. As 3D models make their way into laboratories there is a need to develop characterisation techniques that are sensitive enough to monitor the cells in real time and without the need for chemical labels. Impedance spectroscopy has been shown to address both of these challenges, but there has been little research into the full impedance spectrum and how the different components of the system affect the impedance signal. Here we investigate the impedance of human fibroblast cells in 2D and 3D collagen gel cultures across a broad range of frequencies (10 Hz to 5 MHz) using a commercial well with in-plane electrodes. At low frequencies in both 2D and 3D models it was observed that protein adsorption influences the magnitude of the impedance for the cell-free samples. This effect was eliminated once cells were introduced to the systems. Cell proliferation could be monitored in 2D at intermediate frequencies (30 kHz). However, the in-plane electrodes were unable to detect any changes in the impedance at any frequency when the cells were cultured in the 3D collagen gel. The results suggest that in designing impedance measurement devices, both the nature and distribution of the cells within the 3D culture as well as the architecture of the electrodes are key variables. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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