Starch phosphorylation plays an important role in starch biosynthesis
| Autor: | Luisa M. Trindade, Annemarie Dechesne, Richard G. F. Visser, Xuan Xu, Dianka C.T. Dees, Xing-Feng Huang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Freeze-thaw stability Polymers and Plastics Starch Starch metabolism Mutant 03 medical and health sciences chemistry.chemical_compound Laboratorium voor Plantenveredeling Amylose Materials Chemistry Phosphorylation Glucan Solanum tuberosum chemistry.chemical_classification PBR Biobased Economy Starch phosphate content Storage starch Organic Chemistry food and beverages PE&RC Phosphate Starch biosynthesis Plant Breeding 030104 developmental biology Water dikinase chemistry Biochemistry PBR Bio-based Economy EPS |
| Zdroj: | Carbohydrate Polymers 157 (2017) Carbohydrate Polymers, 157, 1628-1637 |
| ISSN: | 1879-1344 0144-8617 |
| Popis: | Starch phosphate esters are crucial in starch metabolism and render valuable functionality to starches for various industrial applications. A potato glucan, water dikinase (GWD1) was introduced in tubers of two different potato genetic backgrounds: an amylose-containing line Kardal and the amylose-free mutant amf. In both backgrounds, this resulted in two contrasting effects, a number of plants showed higher phosphate content compared to the respective control, while others lines exhibited lower phosphate content, thereby generating two series of starches with broad-scale variation in phosphate content. The results of systematic analyses on these two series of starches revealed that starch phosphate content strongly influenced starch granule morphology, amylose content, starch fine structure, gelatinization characteristics and freeze-thaw stability of starch gels. Further analyses on the expression level of genes involved in starch metabolism suggested that starch phosphorylation regulates starch synthesis by controlling the carbon flux into starch while simultaneously modulating starch-synthesizing genes. |
| Databáze: | OpenAIRE |
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