Rapid detection of point mutations in Plasmodium falciparum genes associated with antimalarial drugs resistance by using High-Resolution Melting analysis
| Autor: | L. Rabarijaona, Valérie Andriantsoanirina, Didier Menard, Arsène Ratsimbasoa, Rémy Durand, Christiane Bouchier, Magali Tichit, Vincent Lascombes, Jonathan Hoffman |
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| Rok vydání: | 2009 |
| Předmět: |
Microbiology (medical)
Hot Temperature Plasmodium falciparum Drug Resistance Nucleic Acid Denaturation Microbiology Polymerase Chain Reaction Sensitivity and Specificity High Resolution Melt DNA sequencing law.invention Nucleic acid thermodynamics Antimalarials Parasitic Sensitivity Tests law parasitic diseases Genotype Animals Humans Point Mutation Transition Temperature Molecular Biology Genotyping Polymerase chain reaction biology Point mutation DNA Protozoan biology.organism_classification Molecular biology |
| Zdroj: | Journal of microbiological methods. 78(2) |
| ISSN: | 1872-8359 |
| Popis: | We have developed a High-Resolution DNA Melting method to detect mutations related to Plasmodium falciparum resistance. This method is based on real-time PCR followed by High Resolution Melting ramping from 67 degrees C to 80 degrees C with fluorescence data acquisition set at 0.1 degrees C increments. The accuracy of the technique was assessed using 177 P. falciparum clinical isolates and two reference strains. Results perfectly matched those obtained by DNA sequencing for some important genetic markers of P. falciparum resistance. This technique could be of great value for epidemiological studies, especially in developing countries. |
| Databáze: | OpenAIRE |
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