MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma
| Autor: | Haisu Dai, Yujun Zhang, Yu You, Lian-Hua Bai, Haowei Chen, Aigang Yang, Jiaxin Tan, Ping Bie, Xuejun Xu |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Pathology CD3 Complex Galectin 1 T-Lymphocytes medicine.medical_treatment Apoptosis 0302 clinical medicine Liver Neoplasms hepatocellular carcinoma Middle Aged Prognosis Interleukin-10 Gene Expression Regulation Neoplastic Cytokine Liver Oncology 030220 oncology & carcinogenesis Hepatocellular carcinoma Galectin-1 Cytokines Female hepatic stellate cells Research Paper medicine.medical_specialty Carcinoma Hepatocellular 03 medical and health sciences Immune system Immune privilege Cell Line Tumor microRNA otorhinolaryngologic diseases medicine galectin-1 Humans Aged Oncogene business.industry Gene Expression Profiling medicine.disease Actins MicroRNAs 030104 developmental biology Hepatic stellate cell Cancer research Interferons miRNA-22 K562 Cells business |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.10981 |
| Popis: | // Yu You 1 , Jia-Xin Tan 1 , Hai-Su Dai 1 , Hao-Wei Chen 1 , Xue-Jun Xu 1 , Ai-Gang Yang 1 , Yu-Jun Zhang 1 , Lian-Hua Bai 1 , Ping Bie 1 1 Department of Hepatobiliary Surgery Institute, South Western Hospital, Third Military Medical University, Chongqing 400038, China Correspondence to: Ping Bie, email: bieping@medmail.com.cn Lian-Hua Bai, email: 870426131@qq.com Keywords: hepatocellular carcinoma, hepatic stellate cells, galectin-1, miRNA-22 Received: April 14, 2016 Accepted: July 10, 2016 Published: August 1, 2016 ABSTRACT Hepatic stellate cells (HSCs) induce immune privilege and promote hepatocellular carcinoma (HCC) by suppressing the immune system. On the other hand, galectin-1 and miRNA-22 (miR-22) are dysregulated in HCC and serve as prognostic indicators for patients. In this study, therefore, we measured galectin-1 and miR-22 expression in HSCs isolated from HCC tissues (Ca-HSCs), and in normal liver tissues (N-HSCs) as a control. We also investigated the apoptosis rate among T cells and the production of cytokines (IFN-γ and IL-10) in HSCs co-cultured with T cells. And we used immunohistochemical staining to tested for correlation between galectin-1 expression, CD3 expression and clinicopathological features in 162 HCC patients. Our results showed that galectin-1 expression was much higher in Ca-HSCs than in N-HSCs. Overexpression of galectin-1 promoted HSC-induced T cell apoptosis and cytokine production (IFN-γ and IL-10), while miR-22 expression inhibited it. Galectin-1 expression correlated negatively with miR-22 expression in HSCs. High galectin-1 and low CD3 expression levels were associated with poor prognosis in HCC patients. These results suggest that the immunosuppressive microenvironment promoted by HSC-derived galectin-1 in HCC can be inhibited by miR-22. Galectin-1 and miR-22 could potentially serve as prognostic markers and therapeutic targets in HCC. |
| Databáze: | OpenAIRE |
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