In vitro and in vivo effects of rat kidney vascular endothelial cells on osteogenesis of rat bone marrow mesenchymal stem cells growing on polylactide-glycoli acid (PLGA) scaffolds
| Autor: | Bai-Can Yang, Hongchen Sun, Ying-Ying Guo, Guangxiang Zang, Zhe Qu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
CD31
lcsh:Medical technology Endothelium Polymers Cellular differentiation Osteocalcin Biomedical Engineering Cell Culture Techniques Bone Marrow Cells Bone tissue Kidney Models Biological Biomaterials Tissue engineering Polylactic Acid-Polyglycolic Acid Copolymer Osteogenesis medicine Animals Radiology Nuclear Medicine and imaging Lactic Acid Cell Proliferation Osteoblasts Radiological and Ultrasound Technology Tissue Engineering Chemistry Regeneration (biology) Research Mesenchymal stem cell Kidney metabolism Endothelial Cells Cell Differentiation Mesenchymal Stem Cells General Medicine Alkaline Phosphatase Coculture Techniques Cell biology Rats Platelet Endothelial Cell Adhesion Molecule-1 medicine.anatomical_structure lcsh:R855-855.5 Immunology Endothelium Vascular Polyglycolic Acid |
| Zdroj: | BioMedical Engineering OnLine, Vol 6, Iss 1, p 41 (2007) BioMedical Engineering |
| Popis: | It is well established that vascularization is critical for osteogenesis. However, adequate vascularization also remains one of the major challenges in tissue engineering of bone. This problem is further accentuated in regeneration of large volume of tissue. Although a complex process, vascularization involves reciprocal regulation and functional interaction between endothelial and osteoblast-like cells during osteogenesis. This prompted us to investigate the possibility of producing bone tissue both in vitro and ectopically in vivo using vascular endothelial cells because we hypothesized that the direct contact or interaction between vascular endothelial cells and bone marrow mesenchymal stem cells are of benefit to osteogenesis in vitro and in vivo. For that purpose we co-cultured rat bone marrow mesenchymal stem cells (MSC) and kidney vascular endothelial cells (VEC) with polylactide-glycolic acid scaffolds. In vitro experiments using alkaline phosphatase and osteocalcin assays demonstrated the proliferation and differentiation of MSC into osteoblast-like cells, especially the direct contact between VEC and MSC. In addition, histochemical analysis with CD31 and von-Willebrand factor staining showed that VEC retained their endothelial characteristics. In vivo implantation of MSC and VEC co-cultures into rat's muscle resulted in pre-vascular network-like structure established by the VEC in the PLGA. These structures developed into vascularized tissue, and increased the amount and size of the new bone compared to the control group (p < 0.05). These results suggest that the vascular endothelial cells could efficiently stimulate the in vitro proliferation and differentiation of osteoblast-like cells and promote osteogenesis in vivo by the direct contact or interaction with the MSC. This technique for optimal regeneration of bone should be further investigated. |
| Databáze: | OpenAIRE |
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