High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates
| Autor: | Mizuki Hino, Hitoshi Hashimoto, Misaki Niu, Norihito Shintani, Yuichiro Naka, Katsumasa Fujita, Ken-ichi Inoue, Atsuko Hayata-Takano, Takanobu Nakazawa, Toshio Matsuda, Kaoru Seiriki, Masato Tanuma, Hisato Igarashi, Yasuto Kunii, Masahiko Takada, Kazuki Nagayasu, Ryota Hashimoto, James A. Waschek, Atsushi Kasai, Takeshi Hashimoto, Wiebke Schulze, Shiori Uezono, Takeharu Nagai, Shun Yamaguchi, Akemichi Baba, Hirooki Yabe, Yukio Ago, Kenji F. Tanaka, Junya Matsumoto, Kazuhiro Takuma |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Neuroimaging Group comparison Mice 03 medical and health sciences Functional brain Imaging Three-Dimensional biology.animal Image Processing Computer-Assisted Neurites Animals Humans Tomography Image resolution Aged 80 and over Microscopy Postmortem brain biology General Neuroscience Brain Marmoset Callithrix 030104 developmental biology Microscopy Fluorescence Scalability Female Neuroscience |
| Zdroj: | Neuron. 94:1085-1100.e6 |
| ISSN: | 0896-6273 |
| Popis: | Subcellular resolution imaging of the whole brain and subsequent image analysis are prerequisites for understanding anatomical and functional brain networks. Here, we have developed a very high-speed serial-sectioning imaging system named FAST (block-face serial microscopy tomography), which acquires high-resolution images of a whole mouse brain in a speed range comparable to that of light-sheet fluorescence microscopy. FAST enables complete visualization of the brain at a resolution sufficient to resolve all cells and their subcellular structures. FAST renders unbiased quantitative group comparisons of normal and disease model brain cells for the whole brain at a high spatial resolution. Furthermore, FAST is highly scalable to non-human primate brains and human postmortem brain tissues, and can visualize neuronal projections in a whole adult marmoset brain. Thus, FAST provides new opportunities for global approaches that will allow for a better understanding of brain systems in multiple animal models and in human diseases. |
| Databáze: | OpenAIRE |
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