Enhanced antitumor activity against melanoma cancer cells by nitric oxide release and photosensitized generation of singlet oxygen from ruthenium complexes
| Autor: | Marcelo Dias Baruffi, Daniel R. Callejon, Leonardo D. Slep, Roberto Santana da Silva, Claure N. Lunardi, Mario Sérgio Pereira Marchesi, Loyanne Carla Barbosa Ramos, Lusiane Maria Bendhack |
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| Rok vydání: | 2016 |
| Předmět: |
inorganic chemicals
Pyrazine chemistry.chemical_element MELANOMA 010402 general chemistry Photochemistry 01 natural sciences ANTITUMOR AGENTS TOXICITY Nitric oxide Inorganic Chemistry chemistry.chemical_compound NITROGEN OXIDES Photosensitizer SINGLET OXYGEN 010405 organic chemistry Singlet oxygen RUTHENIUM Otras Ciencias Químicas Ciencias Químicas 0104 chemical sciences Ruthenium chemistry Apoptosis Cell culture Phototoxicity CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | We have investigated the synergistic effect of nitric oxide and singlet oxygen originating from stimulated ruthenium complexes and their photodynamic action against a melanoma cancer cell line. In aqueous solution, the photosensitizer [Ru(NH3)5pz]2+(I; pz = pyrazine) and the nitrosylruthenium complex trans-[RuCl([15]aneN4)NO]2+(II; [15]aneN4= 1,4,8,12-tetrazacyclopentadecane) can produce singlet oxygen and nitric oxide, respectively. The phototoxicity of complex I does not correlate with the singlet oxygen quantum yields unless it is mediated by nitric oxide release from complex II. This effect is around 70 and 50 % higher than the effect prompted by compounds I and II, respectively, in isolation in the presence of light. It was found that the number of hypodiploid cells in the melanoma cancer cells increased, which indicates that a mixture of compounds I and II induces apoptosis of tumorigenic cell lines. Both singlet oxygen and nitric oxide produced by the ruthenium complexes enhance the photodynamic efficacy in melanoma cancer cells. Fil: Ramos, Loyanne C. B.. Universidade de Sao Paulo; Brasil Fil: Marchesi, Mario Sérgio Pereira. Universidade de Sao Paulo; Brasil Fil: Callejon, Daniel. Universidade de Sao Paulo; Brasil Fil: Baruffi, Marcelo Dias. Universidade de Sao Paulo; Brasil Fil: Lunardi, Claure N.. Universidade do Brasília; Brasil Fil: Slep, Leonardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina Fil: Bendhack, Lusiane M.. Universidade de Sao Paulo; Brasil Fil: da Silva, Roberto Santana. Universidade de Sao Paulo; Brasil |
| Databáze: | OpenAIRE |
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