Lipid mono- and bilayer supported on polymer films: composite polymer-lipid films on solid substrates
| Autor: | Robert Tampé, M. Kühner, Erich Sackmann |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Lipid Bilayers
Molecular Sequence Data Biophysics Acrylic Resins Diffusion chemistry.chemical_compound Aphthovirus Capsid Monolayer Organic chemistry Lipid bilayer phase behavior Amino Acid Sequence Lipid bilayer Antigens Viral chemistry.chemical_classification Viscosity Bilayer Polymer Permeation Models Theoretical Kinetics Monomer Spectrometry Fluorescence Chemical engineering chemistry Liposomes Capsid Proteins Wetting Dimyristoylphosphatidylcholine Mathematics Research Article |
| Zdroj: | Biophysical journal. 67(1) |
| ISSN: | 0006-3495 |
| Popis: | We report the deposition of lipid monolayers and bilayers on polyacrylamide films deposited by radical chain reaction onto solid substrates in aqueous solutions. Polymer films of various degrees of monomer density and cross-linking are prepared. Lateral diffusion and fluorescent probe permeation measurements yield insight into the continuity of the lipid layers and show that monolayers exposed to air are much less sensitive towards polymer heterogeneities than bilayers below water, which is explained in terms of the wetting laws. The diffusion studies of lipid and lipopeptide probes yield absolute values of the frictional coefficients between the lipid layer and the polymer films and allow one to estimate the surface viscosity of the polymer film. The potential applications of supported membranes on soft thin polymer films for the preparation of biofunctionalized surfaces or biocompatible receptive surfaces for biosensors are discussed. |
| Databáze: | OpenAIRE |
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