Induction of Both Local Immune Response in Mice and Protection in a Rabbit Model by Intranasal Immunization with Modified Vaccinia Ankara Virus Expressing a Secreted Form of Bovine Herpesvirus 1 Glycoprotein D
| Autor: | Carlos Rodolfo Federico, Osvaldo Zabal, María Soledad Esusy, Gabriela Calamante, María Paula Del Médico Zajac, Alejandro Rafael Valera, Flavia Zanetti |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Modified vaccinia Ankara viruses medicine.medical_treatment Immunology Vaccinia virus Herpesvirus Vaccines Biology Antibodies Viral complex mixtures Virus 03 medical and health sciences Viral Proteins 0302 clinical medicine Immune system Virology medicine Animals 030212 general & internal medicine Administration Intranasal Herpesvirus 1 Bovine Drug Carriers Mice Inbred BALB C Vaccines Synthetic Immunogenicity Herpesviridae Infections biology.organism_classification Bovine herpesvirus 1 Disease Models Animal 030104 developmental biology Immunization biology.protein Molecular Medicine Female Rabbits Antibody Adjuvant |
| Zdroj: | Viral immunology. 30(1) |
| ISSN: | 1557-8976 |
| Popis: | In this study, we evaluated the immunogenicity and efficacy of mucosal delivery of a recombinant modified vaccinia Ankara virus (MVA) expressing the secreted version of bovine herpesvirus type 1 (BoHV-1) glycoprotein D (MVA-gDs) without addition of adjuvant in two animal models. First, we demonstrated the capability of MVA-gDs of inducing both local and systemic anti-gD humoral immune response after intranasal immunization of mice. Then, we confirmed that two doses of MVA-gDs administered intranasally to rabbits induced systemic anti-gD antibodies and conferred protection against BoHV-1 challenge. Our results show the potential of using MVA as a vector for the rational design of veterinary vaccines capable of inducing specific and protective immune responses both at local and systemic level. |
| Databáze: | OpenAIRE |
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