Endothelial Cytosolic Proteins Bind to the 3′ Untranslated Region of Endothelial Nitric Oxide Synthase mRNA: Regulation by Tumor Necrosis Factor Alpha
| Autor: | L. Sánchez de Miguel, S. Casado, Javier Alonso, M Montón, Antonio López-Farré |
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| Rok vydání: | 1997 |
| Předmět: |
Untranslated region
MRNA destabilization Molecular Sequence Data RNA-binding protein Regulatory Sequences Nucleic Acid Biology Cytosol Enos Animals RNA Messenger Molecular Biology Cells Cultured Messenger RNA Base Sequence Tumor Necrosis Factor-alpha Three prime untranslated region RNA-Binding Proteins Cell Biology biology.organism_classification Molecular biology Nitric oxide synthase biology.protein Cattle Tumor necrosis factor alpha Endothelium Vascular Nitric Oxide Synthase Polyribonucleotides Research Article |
| Zdroj: | Scopus-Elsevier ResearcherID |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.17.10.5719 |
| Popis: | Changes in endothelial nitric oxide synthase (eNOS) expression may be involved in the endothelium-dependent vasorelaxation dysfunction associated with several vascular diseases. In the present work, we demonstrate that eNOS mRNA contains a previously undescribed cis element in the 3' untranslated region (3' UTR). A U+C-rich segment in the 3' UTR is critical in complex formation with bovine aortic endothelial cell cytosolic proteins. Tumor necrosis factor alpha (TNF-alpha), which destabilizes eNOS mRNA, increased the binding activity of the cytosolic proteins in a time-dependent manner. These data suggest that endothelial cytosolic proteins bind to the 3' UTR of eNOS mRNA. These proteins may play a role in TNF-alpha-induced eNOS mRNA destabilization. |
| Databáze: | OpenAIRE |
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