Detection of HCV genome in peripheral blood mononuclear cells of Iranian seropositive and HCV RNA negative in plasma of patients with beta‐thalassemia major: Occult HCV infection
| Autor: | Roya Kahyesh‐Esfandiary, Zohreh-Azita Sadigh, Farah Bokharaei-Salim, Tahereh Donyavi, Alireza Najafi, Atousa Fakhim, Mohammad-Navid Bastani, Maryam Esghaei |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male Blood transfusion Adolescent Genotyping Techniques Hepatitis C virus medicine.medical_treatment Thalassemia Hepacivirus Iran medicine.disease_cause Polymerase Chain Reaction Peripheral blood mononuclear cell Plasma Young Adult 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Virology Blood plasma Prevalence medicine Humans 030212 general & internal medicine Child NS5B biology business.industry beta-Thalassemia virus diseases Hepatitis C Middle Aged medicine.disease digestive system diseases Cross-Sectional Studies Infectious Diseases chemistry DNA Viral Leukocytes Mononuclear biology.protein RNA Viral Female 030211 gastroenterology & hepatology Antibody business |
| Zdroj: | Journal of Medical Virology. 91:107-114 |
| ISSN: | 1096-9071 0146-6615 |
| DOI: | 10.1002/jmv.25279 |
| Popis: | Beta (β) thalassemia major is a genetic blood disorder with a deficiency in the hemoglobin beta chain, requiring blood transfusion therapy. Multiple blood transfusions increase the risk of transmitting blood-borne infections. The aim of this study is to determine the frequency of hepatitis C virus (HCV) infection in Iranian individuals with β-thalassemia major. A total of 164 patients with β-thalassemia major were recruited for this study. HCV RNA testing was done on plasma and peripheral blood mononuclear cells (PBMCs) from the HCV seropositive samples (with reverse transcriptase-nested polymerase chain reaction [PCR] method using primers from the 5'-untranslated region [UTR]), and all HCV RNA positive samples were genotyped by the restriction fragment length polymorphism assay. For confirmation of the HCV genotyping in PBMCs of occult HCV infection [OCI]-positive patients, the PCR products of two different regions of HCV (5'-UTR and nonstructural protein 5B [NS5B]) were sequenced. Of 164 patients, 29.3% were positive for anti-HCV antibodies, and HCV RNA was detected in the plasma specimens of 13.4% patients and in the PBMC samples of 15.2% participants. The genomic HCV-RNA was detected in PBMC samples in 3 (6.3%) of the total 48 individuals who were HCV seropositive, and plasma HCV-RNA negative (occult HCV infection). The subtypes of HCV in the plasma and PBMC samples of three participants were not identical. This study shows that among this group of Iranian patients with β-thalassemia major, 13.4% had active HCV infection and 6.3% had occult HCV infection as evidenced by HCV RNA detected in PBMC specimens. Therefore, the design of a prospective study that focuses on the diagnosis of OCI can be very valuable and provide more information. |
| Databáze: | OpenAIRE |
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