Label-free volumetric quantitative imaging of human osteosarcoma cells by hyperspectral coherent anti-Stokes Raman scattering
| Autor: | Karuna, A., Masia, Francesco, Wiltshire, Marie, Errington, Rachel, Borri, Paola, Langbein, Wolfgang, Periasamy, Ammasi, So, Peter T. C., Konig, Karsten |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Quantitative imaging
Materials science Coherent Raman scattering Resolution (electron density) Hyperspectral imaging Quantitative determination Coherent anti-Stokes Raman scattering microscopy symbols.namesake Nuclear magnetic resonance CARS label-free imaging Microscopy symbols CARS Chemical composition Raman scattering label-free imaging Label free |
| Zdroj: | Proceedings of SPIE: Multiphoton Microscopy in the Biomedical Sciences XIX, 10882 |
| ISSN: | 0277-786X 2410-9045 |
| Popis: | Quantitative determination of the chemical composition of unstained samples, non-invasively, with high three- dimensional spatio-temporal resolution, will accelerate progress in cell biology. The current state of the art in bioimaging is dominated by either chemically non-specific or invasive methods. In this work, we demonstrate label-free, non-invasive quantitative volumetric imaging of human osteosarcoma cells using coherent anti-Stokes Raman scattering microscopy. A data analysis method developed in-house was applied to represent the chemical composition of the cells as volumetric three-dimensional images indicating water, proteins, DNAP (mixture of DNA and proteins), and lipids, and to determine the dry masses of the organic components with picogram resolution. |
| Databáze: | OpenAIRE |
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